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Understanding Nonhomologous DNA End Joining: Novel Insights Into Mechanism and Regulation
This study has provided new insights into the mechanistic details of NHEJ
and showed that DNA sequence around the break site can be a factor in determining which
proteins involved in NHEJ might be recruited with a higher ...
Mechanistic Insights Into Heat Shock Protein 90 (Hsp90) Trans-splicing in Giardia lamblia
Hsp90 gene is conserved and encoded by a single ORF with none to many cis-spliced introns across the biological kingdom. Previous studies from our lab have shown that Hsp90 gene in Giardia lamblia has a split nature having ...
Role of Escherichia coli UvrD helicase in resolving G-quadruplex DNA structures
G-quadruplex (G4) secondary structures have been implicated in various biological processes,
including gene expression, DNA replication and telomere maintenance. However,
unresolved G4 structures impede replication ...
Kinetic, Catalytic and Epigenetic Properties of HpyAXVII DNA Methyltransferase – a Phasevarion in the Human Pathogen Helicobacter pylori
Helicobacter pylori is the leading cause of gastric cancer in human beings. It is a helix-shaped, Gram-negative, motile bacteria that colonizes the gastric mucosa and eventually the gastric epithelium. It has an overabundance ...
Determination of significance of SUMOylation of the Smc5/6 complex
The fascination of scientists to the Smc5/6 complex began in the 1990s, with the characterization of UV and γ radiation-sensitive rad18 mutant and its identification as a component of a novel Structural Maintenance of ...
Biochemical characterization of UvrD helicase and its 1 interplay with DNA mismatch repair proteins in 2 Neisseria gonorrhoeae
UvrD helicase belongs to type I superfamily of helicases. It participates in Nucleotide Excision Repair (NER) and DNA Mismatch Repair (MMR). It also participates in the rolling circle replication. It has been shown that ...
Investigation of RAG1 in lymphoid and brain cells: Understanding mechanism and regulation
In summary, we show that RAG1 expression is regulated through a novel miRNA (miR-
29c) mediated mechanism and establish that as an additional mode of RAG1 regulation.
Studies described in the thesis reveal that among the ...
Development and Characterization of a potent Tumor Necrosis Factor-alpha blocking agent
Tumor necrosis factor-α (TNFα), one of the major pro-inflammatory cytokines, plays a pivotal role in an effective inflammation process and immune response. TNFα interacts with two different receptors, TNFR1 and TNFR2, which ...
Conservation and divergence of the nucleotide excision repair pathway: Comparison of UvrB and UvrC proteins from Mycobacterium tuberculosis and Escherichia coli reveal significant differences in their functional properties
This thesis is presented in three chapters divided by topics. Chapter I will begin with an overview of literature regarding different types of DNA damage, DNA repair pathways in bacteria, properties of E. coli UvrABC ...
Production of taxol from Lasiodiplodia theobromae and biochemical characterization of its 10-deacetylbaccatin III-10-O-acetyltransferase enzyme and evaluation of anticancer activity of enzymatically synthesized baccatin III
Taxol (paclitaxel) originally identified from the inner bark of Taxus brevifolia, is a clinically approved anticancer drug for the treatment of various kinds of cancer, particularly ovarian cancer, breast cancer and Kaposi's ...