Biochemistry (BC)
Recent Submissions
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Studies on the roles of rffG and rfbB encoding dTDP-glucose 4,6- dehydratase in Salmonella Typhimurium
Salmonellosis is a major health concern which causes significant morbidity and mortality worldwide. Infection with Salmonella Typhimurium leads to self-limiting diarrhea in healthy individuals and invasive disease in ... -
Roles of Protein Acetylation in the Human Pathogen Helicobacter pylori
Helicobacter pylori is a gram-negative epsilon proteobacterium infecting half of the world population. H. pylori is a naturally competent bacterium with a huge repertoire of Restriction-Modification (RM) systems. The ... -
Regulation of Flagellar Motility in Giardia lamblia and Trichomonas vaginalis
Protozoan pathogens are responsible for infections that are highly prevalent, especially in developing and under-developed countries. Flagellar motility is exhibited by a variety of organisms ranging from bacteria to certain ... -
The dual-targeting of Saccharomyces cerevisiae Pso2 into the nucleus and mitochondria is regulated by post-translational modifications: Implications for organelle-specific DNA repair
The repair of DNA interstrand crosslinks (ICLs) involves the concerted action of multiple DNA repair pathways. In eukaryotes, ICLs are sensed and repaired by replication-coupled or replication-independent mechanisms in ... -
Understanding the Mechanism of Noncanonical Functions of RAGs in Lymphoid Cancer and Glioblastoma
Recombination activating genes (RAGs) composed of RAG1 and RAG2, are the endonuclease involved in V(D)J recombination, which is critical for adaptive immunity in mammals. Activation-induced cytidine deaminase (AID), expressed ... -
Regulation of carbon metabolism in Pichia pastoris by Mxr1p and identification of novel regulators
Pichia pastoris (a.k.a. Komagataella phaffii), a widely used yeast for recombinant protein production, is cultured under a variety of conditions ranging from shake flasks to large bioreactors employing minimal, chemically ... -
Role of mammalian RAD51 paralogs in replication stress response and genome maintenance
The focus of my research has been to understand the molecular mechanisms underlying the role of mammalian RAD51 paralogs in genome maintenance and tumor suppression. The investigation carried out during this study has ... -
Molecular and biochemical studies on components of transcription regulation, tropane alkaloid biosynthesis and signal transduction in the medicinal plant Datura metel L.
Tropane alkaloids (TA) are among the oldest medicines known to humankind as they possess hallucinogenic and poisonous properties. Datura metel, an herbaceous plant, belongs to the family Solanaceae whose members are well ... -
Understanding mRNA fate regulation by RGG motif protein, Sbp1
Cellular events that rely on translation regulation has been well established, however the molecular details of factors involved in bringing translation control remains inadequately explored. RNA binding proteins form ... -
Conservation and divergence of the nucleotide excision repair pathway: Comparison of UvrB and UvrC proteins from Mycobacterium tuberculosis and Escherichia coli reveal significant differences in their functional properties
This thesis is presented in three chapters divided by topics. Chapter I will begin with an overview of literature regarding different types of DNA damage, DNA repair pathways in bacteria, properties of E. coli UvrABC ... -
Development and Characterization of a potent Tumor Necrosis Factor-alpha blocking agent
Tumor necrosis factor-α (TNFα), one of the major pro-inflammatory cytokines, plays a pivotal role in an effective inflammation process and immune response. TNFα interacts with two different receptors, TNFR1 and TNFR2, which ... -
Production of taxol from Lasiodiplodia theobromae and biochemical characterization of its 10-deacetylbaccatin III-10-O-acetyltransferase enzyme and evaluation of anticancer activity of enzymatically synthesized baccatin III
Taxol (paclitaxel) originally identified from the inner bark of Taxus brevifolia, is a clinically approved anticancer drug for the treatment of various kinds of cancer, particularly ovarian cancer, breast cancer and Kaposi's ... -
Functional characterization of Salmonella Typhimurium encoded Cold Shock Protein E during stress responses, biofilm formation and virulence
Overall, this study reports novel functional roles of S. Typhimurium encoded CspE during bile resistance, biofilm formation and in vivo virulence. It also establishes importance of an uncharacterised protein YciF, implicating ... -
Insights into the Differential Cytotoxicity of Abrin and Design of a Vaccine Candidate against the Toxin
Ribosome inactivating proteins (RIPs) from plants, fungi or bacteria are extremely lethal due to their N-glycosidase activity on eukaryotic ribosomal RNA. As the name indicates, these toxins inactivate ribosomes; thereby ... -
A role for the cohesin complex in subtelomeric gene silencing in Saccharomyces cerevisiae
Chromatin is a dynamic structure which reorganizes to support numerous chromosomal processes. Various histone and non-histone proteins are involved in chromatin organization. One such group of non-histone proteins, known ... -
New insights into the role of condensin in Saccharomyces cerevisiae
Structural Maintenance of Chromosomes (SMC) complexes are highly conserved from prokaryotes to eukaryotes. Saccharomyces cerevisiae has six SMC proteins which form three SMC-complexes in association with non-SMC subunits ... -
Regulation of RecA nucleoprotein filament stability by RecX and the effects of RecA-membrane interaction on the activities of RecA in mycobacteria
E. coli RecA is a multifunctional protein known to be associated with the cell membrane, forming foci often located at the cell poles, which gets redistributed along the length of the cell during SOS response. Several lines ... -
Mechanistic insights into the replication of Sesbania mosaic virus
The present thesis deals with the mechanism of replication of Sesbania mosaic virus (SeMV), which belongs to the genus sobemoviruses. Sobemoviruses are single stranded positive sense RNA viruses which infect both mono and ... -
Uncovering of a novel non-taxane anticancer secondary metabolite from Taxol-producing endophytic fungus: Optimization of culture parameters and whole-genome sequence analysis for improved taxane production biosynthesis.
Cancer-related mortalities and morbidities have proven to be a great health burden for human society with immense economic implications. With uncontrolled proliferation of cells as the fundamental principle underlying its ... -
Identification and Biochemical Characterization of Novel Inhibitors of Nonhomologous End Joining: Implications in DNA Double-Strand Break Repair and Cancer Therapy
To summarize, in the present study, we have synthesized and characterized three novel NHEJ inhibitors targeting DNA Ligase IV, with several fold better efficacy compared to SCR7 and thus potential to be developed as cancer ...
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