Understanding Nonhomologous DNA End Joining: Novel Insights Into Mechanism and Regulation

Abstract

This study has provided new insights into the mechanistic details of NHEJ and showed that DNA sequence around the break site can be a factor in determining which proteins involved in NHEJ might be recruited with a higher efficiency. We have also generated a KU70-mutant Nalm6 cell line (N6-Ku70-2) using CRISPR-Cas9 genome editing technology and explored the differences between these cells and WT Nalm6 cells in terms of cell proliferation and DNA repair potential. Finally, we have investigated the importance of RNASEH2A/RNASEH2B expression in the maintenance of proper DSB repair and cellular viability in T-ALL cells.