Conservation and divergence of the nucleotide excision repair pathway: Comparison of UvrB and UvrC proteins from Mycobacterium tuberculosis and Escherichia coli reveal significant differences in their functional properties

Abstract

This thesis is presented in three chapters divided by topics. Chapter I will begin with an overview of literature regarding different types of DNA damage, DNA repair pathways in bacteria, properties of E. coli UvrABC subunits and their DNA damage-detection capabilities. Thereafter, the chapter will briefly discuss the biological significance, cellular responses as well as the components of NER pathway in M. tuberculosis. Chapter II and III follow a similar organization, presented in the following order: summary of the results, introduction and aims of the study, results and discussion. Specifically, in Chapter II, the biochemical and functional characterization of wild-type and variants of M. tuberculosis UvrB are presented. Chapter III includes biochemical and functional characterization of wild-type and variants of M. tuberculosis UvrC