Browsing Division of Biological Sciences by Title
Now showing items 770-789 of 1026
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Structural and Functional Characterization of Quadruplex DNA Structures in Human Acetyl-CoA Carboxylase 1 Gene Promoters and Stabilization of G-quadruplex DNA by Carbazole-benzimidazole Conjugates
A number of studies have established that DNA targeting is a successful strategy in anticancer therapy. In recent years, however, the focus has shifted from double-stranded DNA to alternative DNA motifs such as G-quadruplex ... -
Structural and functional insights into hybrid AT-less megaenzyme synthase (NRPS) and DNA-MsDps2 complexes using single particle cryo-EM
Microorganisms, mainly bacteria and fungi, are the producers of structurally diverse, complex organic compounds called as secondary metabolites. These metabolites include polyketides (PKs), non-ribosomal peptides (NRPs), ... -
Structural and functional insights into hybrid AT-less megaenzyme synthase (NRPS) and DNA-MsDps2 complexes using single particle cryo-EM
Microorganisms, mainly bacteria and fungi, are the producers of structurally diverse, complex organic compounds called as secondary metabolites. These metabolites include polyketides (PKs), non-ribosomal peptides (NRPs), ... -
Structural and functional insights of biological macromolecules associated with infectious diseases – single particle cryo-electron microscopy based studies
In the global context, particularly in India, the two most important infectious diseases among many others are Tuberculosis (TB), an age-old disease and COVID-19, a new-age disease. This thesis focuses on some of the key ... -
Structural and Functional Investigation of a Multi-drug Efflux Transporter QacA
The emergence of multi-drug resistance in bacteria is a global health care challenge. One of the effective means of gaining antimicrobial resistance, among superbugs, is through expression of efflux pumps. Quaternary ... -
Structural and functional studies on a small Heat Shock Protein from Entamoeba histolytica
Small Heat Shock Proteins (sHSPs) are molecular chaperones that bind to unfolding proteins during cellular stress to prevent them from aggregation. Once the normal conditions are restored, sHSPs release the bound substrate ... -
Structural and functional studies on DNA damage inducible protein 1 (Ddi1) from protozoa
Structural and functional investigations on DNA-damage inducible protein1 (Ddi1) from Entamoeba histolytica, Trypanosoma cruzi and Toxoplasma gondii have been carried out. Ddi1 belongs to the ubiquitin receptor family of ... -
Structural and Functional Studies on Pyridoxal 5′-Phosphate Dependent Lyases and Aminotransferases
(2017-11-30)The thesis describes structural and functional studies of two PLP-dependent enzymes, diaminopropionate (DAP) ammonia lyase (DAPAL) and N-acetylornithine aminotransferase (AcOAT). The main objective of this work was to ... -
Structural and Functional Studies on Pyridoxal Kinase and Pyridoxal 5′-phosphate Dependent Enzymes
(2018-07-04)Most of the chemical reactions of living cells are catalyzed by protein enzymes. These enzymes are very efficient and display a high degree of specificity with respect to the reaction catalyzed. Cellular activities depend ... -
Structural and Functional Studies on Salmonella typhimurium Propionate Kinase and Photorhabdus luminescens Oxalate Decarboxylase
Acetate and propionate are low molecular mass carbon compounds found abundantly in the soil. Although these compounds have been extensively used as food preservatives because of their ability to inhibit microbial growth, ... -
Structural And Functional Studies On Staphylococcus Aureus Enzymes Involved In L-Lysine Biosynthesis
(2011-05-24)Proteins associated with metabolic pathways have received considerable attention in an effort to understand the molecular details of the complex reactions catalyzed in vivo. Enzymes belonging to these pathways have also ... -
Structural and Functional Studies on the Mycobacterium tuberculosis σ factor σJ
(2018-05-21)Regulation of transcription in prokaryotes is primarily governed at the transcription initiation step. This feature has been extensively characterized in model prokaryotes notably Escherichia coli and Bacillus subtilis. ... -
Structural and Mechanistic Features of Protein Assemblies with Special Reference to Spliceosome
(2017-12-07)Macromolecular assemblies such as the ribosome, spliceosome, polymerases are imperative for cellular functions. The current understanding of these important machineries and many other assemblies at the molecular level is ... -
Structural And Mechanistic Studies On Receptor Protein Tyrosine Phosphatases From Drosophila Melanogaster
(2013-06-20)Protein Tyrosine Phosphatases (PTPs) initiate, modulate and terminate key cellular processes by dephosphorylating phosphotyrosine (pY) residues on signaling proteins. The coordinated action of PTPs with their cognate ... -
Structural and mechanistic studies on Staphylococcal RNA degrading enzymes and multienzyme complexes
RNA degrading enzymes and multi-enzyme complexes govern a variety of cellular processes. The role of these enzymes and multi-enzyme assemblies has been suggested to govern gene expression levels in bacteria with modulations ... -
Structural and Related Studies on Mycobacterial Lectins
(2018-05-09)This thesis is concerned with the first ever X-ray crystallographic and complimentary solution studies on mycobacterial lectins. Lectins, described as multivalent carbohydrate binding proteins of non-immune origin, are ... -
Structural and Related Studies on Mycobacterial RecA and LexA
(2018-06-18)Genetic material of bacteria is subject to damage due to multitudinous factors, both extrinsic and intrinsic in origin. Mechanisms for the maintenance of genomic integrity are thus essential for a bacterium to survive. ... -
Structural and related studies on Mycobacterium tuberculosis pantothenate kinase
Pantothenate kinase (PanK) is a ubiquitous and essential enzyme that catalyses the first step in the universal Coenzyme A (CoA) biosynthetic pathway. In this step pantothenate is converted to phosphopantothentate, which ... -
Structural Characterization of Biological Macromolecules by Random Conical Tilt Pair Experiments
Structural biology deals with determining the structure of biological macromolecules especially proteins, DNA, RNA. The conformational changes in the structure of a macromolecule helps to decipher its function. This ... -
Structural Characterization Of Protein Folding Intermediates
(2013-07-25)