Molecular Biophysics Unit (MBU): Recent submissions
Now showing items 141-160 of 301
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Effect of Ultrasound on Neuronal Network Communication
(2018-05-22)Low intensity and low frequency ultrasound has been shown to modulate ion channel currents, membrane capacitive currents, and as a result, neuronal activity. Ultrasound has been used as a non-invasive way to modulate ... -
Dynamics of Protein Kinases : Its Relationship to Functional Sites and States
(2018-05-22)A cell is a highly complex, ordered, and above all, a robust system. It copes with in-trennel and external uncertainties like heterogeneous stimuli, errors in processing and execution, and changes within and outside the ... -
Structural and Functional Studies on the Mycobacterium tuberculosis σ factor σJ
(2018-05-21)Regulation of transcription in prokaryotes is primarily governed at the transcription initiation step. This feature has been extensively characterized in model prokaryotes notably Escherichia coli and Bacillus subtilis. ... -
Biochemical, Biophysical and Evolutionary Perspectives of Zinc Finger Proteins in Mycobacterium smegmatis
(2018-05-21)Transcription is a major step in expression of genes of a given organism. Due to environmental constrains this step must be regulated in the favour of the sustenance and growth of the organism. Here comes the relevance of ... -
Structural Feature of Prokaryotic Promoters and their Role in Gene Expression
(2018-05-14)Transcription initiation is an important step in the process of gene regulation in prokaryotes. Promoters are stretches of DNA sequence that are present in the upstream region of transcription start sites (TSSs), where RNA ... -
Molecular Characterization of Bacillus Subtilis Oxidoreductases involved in the Bacilysin Synthesis
(2018-05-11)The biosynthetic pathway for the production of the dipeptide antibiotic bacilysin has been the subject of intense research over the past three decades. These studies revealed the role of multiple enzymes in the biosynthesis ... -
Structural Studies on the Role of Hinge involved in Domain Swapping in Salmonella Typhimurium Stationary Phase Survival Protein (SurE) and Sesbania Mosaic Virus Coat Protein
(2018-05-09)A unique mechanism of protein oligomerization is domain swapping. It is a feature found in some proteins wherein a dimer or a higher oligomer is formed by the exchange of identical structural segments between protomers. ... -
Structural Studies on Heat Shock Protein 90 from Dictyostelium Discoideum and Oryza Sativa
(2018-05-09)Molecular chaperones are proteins that interact with and aid in stabilization and activation of other proteins. Chaperones help proteins attain their three dimensional conformation, without forming a part of the final ... -
Structural and Related Studies on Mycobacterial Lectins
(2018-05-09)This thesis is concerned with the first ever X-ray crystallographic and complimentary solution studies on mycobacterial lectins. Lectins, described as multivalent carbohydrate binding proteins of non-immune origin, are ... -
Protein Engineering and Stabilization of HIV-1 Envelope Glycoprotein
(2018-05-09)A number of viral diseases such as Hepatitis B, small pox, measles, rubella and polio have effective vaccines to control or eradicate them. HIV-1 is a lentivirus which infects human immune cells and leads to the disease ... -
Small Heat Shock Proteins from Oryza Sativa and Salmonella Enterica
(2018-05-09)Small heat shock proteins (sHSPs) are a ubiquitous family of molecular chaperones that play a vital role in maintaining protein homeostasis in cells. They are the first line of defence against the detrimental effects of ... -
Spike-Timing-Dependent Plasticity at Excitatory Synapses on the Rat Subicular Pyramidal Neurons
(2018-05-08)The subiculum is a structure that forms a bridge between the hippocampus and the entorhinal cortex (EC) in the brain, and plays a major role in the memory consolidation process. It consists of different types of pyramidal ... -
Structural Studies on Bacterial Adenylosuccinate Lyase and Sesbania Mosaic Virus Protease
(2018-05-08)The three-dimensional structures of biological macromolecules and molecular assemblies are becoming increasingly important with the changing methodologies of drug discovery. The structures aid in understanding of protein ... -
Protein Engineering of HIV-1 Env and Human CD4
(2018-05-01)Since, its discovery over three decades ago, HIV has wrecked havoc worldwide. According to the UNAIDS report 2011, at present 34 million people is living with HIV and AIDS vaccine with broadly neutralizing activity still ... -
Deciphering Structure-Function Relationships in a Two-Subunit-Type GMP Synthetase by Solution NMR Spectroscopy
(2018-04-24)The guanosine monophosphate synthetase (GMPS) is a class I glutamine amidotransferase, involved in the de-novo purine nucleotide biosynthesis. The enzyme catalyzes the biochemical transformation of xantosine (XMP) into ... -
Elucidating the Role of MsRbpA in Rifampicin Tolerance and Transcription Regulation of Mycobacterium Smegmatis
(2018-04-12)RNA polymerase binding protein A (RbpA) was first discovered as a RNA polymerase binding protein from Streptomyces. coelicolor. It was shown to cause rifampicin tolerance to RNA polymerase in vitro and leads to basal level ... -
Structure, Stability and Evolution of Multi-Domain Proteins
(2018-04-11)Analyses of protein sequences from diverse genomes have revealed the ubiquitous nature of multi-domain proteins. They form up to 70% of proteomes of most eukaryotic organisms. Yet, our understanding of protein structure, ... -
Structural Studies on Mycobacterial Aspartic Proteinases and Adenylyl Cyclases
(2018-04-11)Structural investigations on two mycobacterial enzymes were carried out. Tuberculosis still remains a major threat to mankind even though drugs against it have been in use for many decades. The emergence of drug resistant ... -
Conformational Analysis of Designed and Natural Peptides : Studies of Aromatic/Aromatic and Aromatic/Proline Interactions by NMR
(2018-04-05)This thesis describes NMR studies which probe weak interactions between amino acid side chains in folded peptide structures. Aromatic/aromatic interactions between facing phenylalanine residues have been probed in antiparallel ...