Molecular Biophysics Unit (MBU): Recent submissions
Now showing items 161-180 of 301
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Protein NMR Studies of E. Coli IlvN and the Protease-VPg Polyprotein from Sesbania Mosaic Virus
(2018-04-03)Acetohydroxyacid synthase is a multisubunit enzyme that catalyses the first committed step in the biosynthesis of the branched chain amino acids viz., valine, leucine and isoleucine. In order to understand the structural ... -
Mass Spectrometric Deconvolution of Libraries of Natural Peptide Toxins
(2018-03-24)This thesis deals with the analysis of natural peptide libraries using mass spectrometry. In the course of the study, both ribosomal and non-ribosomal classes of peptides have been investigated. Microheterogeneity, ... -
Structural and Evolutionary Analyses of Signalling Proteins with Special Reference to Protein Kinases
(2018-02-27)Cellular response to environmental changes involves a wide repertoire of complex signalling systems often resulting in up and down regulation of various genes. These mechanisms are generally conserved in a variety of ... -
Dissecting the C-DI-GMP Signaling Pathways : Tools and Tales
(2018-02-27)Evaluating aerodynamic noise from aircraft engines is a design stage process, so that it conform to regulations at airports. Aerodynamic noise is also a principal source of structural vibration and internal noise in ... -
Subthreshold Conductances Regulate Theta-Frequency Local Field Potentials and Spike Phase
(2018-02-21)Local field potentials (LFPs), extracellular potentials that reflect localized electrical activity, have long been used as a window to understand the behavioural dependence and mechanistic aspects of brain physiology. A ... -
Structure Determination of Proteins of Unknown Origin by a Marathon MR Protocol and Investigations on Parameters Important for Molecular Replacement Structure Solution
(2018-02-20)Occasionally, crystallisation of proteins works in mysterious ways! One might obtain crystals of a protein of unknown identity in place of the protein for which crystallisation experiments were performed. If the investigator ... -
NMR Solution Structures of Human γC-Crystallin & the Intrinsically Disordered Viral Genome Linked Protein in the Free & Bound Form
(2018-02-14)This thesis describes the tertiary structures and dynamic studies of two protein systems. The first is human γC -crystallin protein, which is present in the nucleus of the human eye lens and the other is the plant viral ... -
Design of Influenza Immunogens by Hemagglutinin (HA) Protein Minimization
(2018-01-31)Influenza virus is a pleiomorphic human pathogen which causes self-limiting respiratory illness lasting one-two weeks in most individuals. However, in immunologically compromised individuals, influenza infection may lead ... -
Structural Studies on DNA Damage Inducible Protein 1 (Ddi1) of Leishmania and the Rotavirus Nonstructural Protein NSP4
(2018-01-17)Structuraj investigations on the Ddi1 (DNA-damage inducible protein 1) of Leishmania major and on the rotavirus nonstructural protein NSP4 were carried out. Ddi1 belongs to the ubiquitin receptor family of proteins. One ... -
A Systems Biology Approach towards Understanding Host Response and Pathogen Adaptation in Latent Tuberculosis Infection
(2018-01-04)Mycobacterium tuberculosis, the etiological agent of tuberculosis, has adapted with the host environment and evolved to survive in harsh conditions in the host. The pathogen has successfully evolved strategies not only to ... -
Role of Lactate and TREK1 Channels in Neuroprotection during Cerebral Ischemia – in Vitro Study in Rat Hippocampus
(2017-12-16)Cerebral ischemia is a highly debilitating condition where shortage of oxygen and glucose leads to profuse cell death. Insufficient blood supply to the brain leads to cerebral ischemia and increase in extracellular lactate ... -
Structural and Mechanistic Features of Protein Assemblies with Special Reference to Spliceosome
(2017-12-07)Macromolecular assemblies such as the ribosome, spliceosome, polymerases are imperative for cellular functions. The current understanding of these important machineries and many other assemblies at the molecular level is ... -
Further Structural Studies on Jacalin and Genomics Search for Mycobacterial and Archeal Lectins
(2017-12-03)This thesis consists of two parts. The first part is concerned with further structural and related studies of jacalin, one of the two lectins found in jack fruit seeds. The second part deals with the search of mycobacterial ... -
Structural and Functional Studies on Pyridoxal 5′-Phosphate Dependent Lyases and Aminotransferases
(2017-11-30)The thesis describes structural and functional studies of two PLP-dependent enzymes, diaminopropionate (DAP) ammonia lyase (DAPAL) and N-acetylornithine aminotransferase (AcOAT). The main objective of this work was to ... -
Structural Studies on Mycobacterium Tuberculosis Peptidyl-tRNA Hydrolase and Ribosome Recycling Factor, Two Proteins Involved in Translation
(2017-11-27)Protein synthesis is a process by which organisms manufacture their proteins that perform various cellular activities either alone or in combination with other similar or different molecules. In eubacteria, protein synthesis ... -
Emergence and Homeostasis of Functional Maps in Hippocampal Neurons
(2017-11-23)Systematic investigations through several experimental techniques have revealed that hippocampal pyramidal neurons express voltage gated ion channels (VGICs) with well-defined gradients along their dendritic arbor. These ... -
The Dynamics of Iron in Miniferritins : A Structure-Function Connection
(2017-11-23)The DNA binding proteins under starvation (Dps) from M. smegmatis are cage-like structures which internalize iron and bind DNA. They provide resistance to the cells from free radical damage, and physically protect the DNA ... -
Structural Studies on Thiolases and Thiolase-like Proteins
(2017-11-21)The genus Mycobacterium comprises some of the most devastating pathogens that infect humans. Mycobacterium tuberculosis causes tuberculosis in humans leading to high morbidity and mortality. The disease is especially ... -
Structural and Conformational Feature of RNA Duplexes
(2017-11-17)In recent years, several interesting biological roles played by RNA have come to light. Apart from their known role in translation of genetic information from DNA to protein, they have been shown to act as enzymes as well ...