Molecular Biophysics Unit (MBU)
Recent Submissions
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Structural and functional characterization of a bacterial TenpIN type III toxin-antitoxin system and its potential as an antibacterial strategy
Bacterial type III toxin-antitoxin (TA) systems were first identified as abortive infection systems wherein a phage infection causes altruistic suicide of the bacterium to prevent bacteriophage spread among its clonal ... -
Understanding Bacterial Heterogeneity in Gene Expression Under Specific and Global Regulatory Control
Intrinsic heterogeneity in natural bacterial populations enable them to escape sudden changes in environment and evolve. Processes like cellular crosstalk, abiotic interactors, host interactions, intracellular signalling ... -
Role of HCN Currents (Ih) in Epileptiform Activity in Subicular Neurons
Subicular burst firing neurons are established as primary instigators of seizure onset and propagation in Temporal Lobe Epilepsy (TLE), a form of focal epilepsy that accounts for approximately 60% of all epilepsy cases in ... -
Characterizing the link between dynamics and function in proteins that violate the structure-function paradigm
The structure-function paradigm states that the three-dimensional structure of a protein is dictated by its amino acid sequence, which influences its function. However, this paradigm fails to explain the behaviour of ... -
Distinct extracellular signatures of active-dendritic chemical and electrical synapses differentially contribute to ripple-frequency oscillations
Extracellular field potentials across brain regions exhibit distinct signatures that depend on several region-specific attributes, including spatiotemporal patterns of afferent inputs, anatomical and physiological properties ... -
Design, Biophysical Characterization, and Serum Epitope Mapping of SARS-CoV-2 RBD Vaccine Immunogens
Embargo up to Jan 9, 2026 -
Structural characterization of Staphylococcal γ-hemolysin in the presence of a lipid environment using single particle cryo-EM
γ-Hemolysin (γ-HL) is a quintessential bicomponent β-pore forming toxin (β-PFT) belonging to the arsenal of “superbug” Staphylococcus aureus. Here, we performed negative staining-TEM and single particle cryo-EM-based ... -
Integrative multiscale modeling and simulations of biomolecules with experimentally testable predictions
This thesis delves into four separate research works related to Integrative multiscale modeling and simulations of biomolecules. The first work deals with the puzzling case of the Pleckstrin Homology Domain (PHD), which ... -
Electrophysiological studies investigating drug effects on hHCN1 channels and Unravelling pore dynamics in Pannexin channels
Hyperpolarization-activated cyclic nucleotide-gated (HCN) ion channel stands out for its unique characteristics, including sensitivity to transmembrane voltage and cyclic nucleotides, as well as its non-selective passage ... -
Characterization of a Staphylococcus aureus multi-enzyme complex involved in the phenotypic switch and host-pathogen interactions
RNA homeostasis is maintained by synchronizing RNA synthesis and RNA degradation. These processes are synchronized to growth conditions and environmental stimuli, thus enabling bacterial survival in diverse environmental ... -
Design and characterization of stabilized mutants of the SARS-CoV-2 Receptor Binding Domain
The global pandemic caused by SARS-CoV-2 has resulted in millions of deaths worldwide, highlighting the urgent need for effective vaccines. The trimeric spike glycoprotein, particularly the receptor-binding domain (RBD), ... -
Structural and functional characterization of human regulator of telomere elongation helicase 1 (RTEL1)
RTEL1 is an ATP-dependent DNA helicase that translocates on the single-stranded DNA (ssDNA) from 5′ to 3′ direction. It is an iron-sulfur (Fe-S) cluster and DEAH motif-containing helicase. RTEL1 has been shown to play ... -
Design of Immunogens Derived from the Conserved Stem of Influenza Virus Hemagglutinin
Embargo up to 15/7/2025 -
Interaction of hnRNPK with RNA and characterization of its phase separation behavior
Heterogenous ribonucleoproteins (hnRNPs) are a class of proteins initially discovered to protect the nascent transcripts before they develop into primary mRNAs. They are involved in almost all the stages of mRNA metabolism, ... -
Determinants of protein stability and function inferred from saturation mutagenesis and next-generation sequencing
Proteins play a central role in all biological processes. Despite a similar underlying composition, they exhibit immense diversity in structures and functions, a subject of great interest. The research presented in this ... -
Plasticity Paradigms: Navigating Protein Conformational Dynamics and Phenotypic Variability in Biological Systems
The complex dynamics and responses of biological systems to environmental cues are shaped by their inherent plasticity and adaptability. This biological plasticity, operational at multiple levels - ranging from the molecular ... -
Deep mutational scanning to enhance immunogenicity and map antibody responses to HIV-1
Embargo upto 15/7/2025 -
Computational investigation of peripheral membrane protein "Pleckstrin homology domain" and its regulation
The peripheral membrane protein repertoire is around 25% composed of the Pleckstrin homology domain (PHD). PHD are component of the multidomain protein and plays the role of an adaptor in recruitment of these proteins ... -
Role of the unstructured N-terminal region and interacting proteins on the function of Mycobacterium tuberculosis σA
A distinctive feature of the bacterial RNA polymerase is the modular organization. The core of this enzyme, comprised of a dimer of the α subunit assembles with the β, β', and ω subunit. The core RNA polymerase enzyme ...
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