Molecular Biophysics Unit (MBU): Recent submissions
Now showing items 121-140 of 299
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Substrate Specificity Determinants of Class III Nucleotide Cyclases
(2018-07-31)Cyclic AMP and cyclic GMP (cAMP and cGMP) are important second messengers in key signal-transduction pathways that mediate various physiological functions in bacteria and eukaryotes. Adenylyl Cyclases (ACs) and Guanylyl ... -
Understanding the Regulatory Steps that Govern the Activation of Mycobacterium Tuberculosis σK
(2018-07-28)A distinctive feature of host-pathogen interactions in the case of Mycobacterium tuberculosis is the asymptomatic latent phase of infection. The ability of the bacillus to survive for extended periods of time in the host ... -
GABAA Receptor Mediated Phasic and Tonic Inhibition in Subicular Pyramidal Neurons
(2018-07-28)GABA is the major inhibitory neurotransmitter in the central nervous system. It binds to two types of receptors –ionotropic GABAA and metabotropic GABAB. The GABAA receptor directly gates a Clionophore that causes ... -
Physiological Interactions between Neuronal Active Conductances And Inositol Trisphosphate Receptors in Neurons and Astrocytes
(2018-07-23)Intricate interactions among constituent components are defining hallmarks of biological systems and sculpt physiology across different scales spanning gene networks to behavioural repertoires. Whereas interactions among ... -
In Silico Perspectives on RNA Structures Modulating Viral Gene Expression and Mechanics of tRNA Transport
(2018-07-19)The repertoire of cellular functions mediated by Ribonucleic acid (RNA) molecules have expanded considerably during the last two decades. The role played by RNA in controlling and regulating gene expression in ... -
Design and Stabilization of Stem Derived Immunogens from HA of Influenza A Viruses
(2018-07-19)Influenza virus belongs to the Orthomyxovirus family of viruses that causes respiratory infection in humans, leading to morbidity and mortality. The mature influenza A virion has an envelope that contains two major surface ... -
Secondary Structures in Proteins : Identification and Analyses
(2018-07-12)Proteins are large biomolecules consisting of one or more long chains of amino acid residues. They perform a vast array of functions within living organisms. In this thesis, we present analyses of different secondary ... -
Development of a Selective Cell-Permeable Protein Phosphatase 1 Inhibitor
(2018-07-10)Selective ‘super-specific’ inhibitors of Protein Phosphatase 1 (PP1) are not available. Several natural product toxins possessing marginal selectivity between PP1 and the closely related Protein Serine/Threonine Phosphatase ... -
Solution Structure Studies on the Effects of Aromatic Interactions and Cross-Strand Disulfide Bonds on Protein Folding
(2018-07-05)The work presented in this thesis focusses primarily on the determination of protein structure at atomic resolution, with NMR spectroscopy as the principle investigative tool. The thesis is divided into four parts. Part I ... -
Structural and Functional Studies on Pyridoxal Kinase and Pyridoxal 5′-phosphate Dependent Enzymes
(2018-07-04)Most of the chemical reactions of living cells are catalyzed by protein enzymes. These enzymes are very efficient and display a high degree of specificity with respect to the reaction catalyzed. Cellular activities depend ... -
Modulation of Protein Stability and Function by Cysteine Mutations and Signal Peptides
(2018-06-23)Chapter 1gives a general introduction to the CXXC motif found in natural proteins. It then reviews the studies where disulphides were engineered in various proteins. The various strategies developed to engineer metal binding ... -
Design and Characterization of HIV-1 ENV Derived Immunogens
(2018-06-21)The Human Immunodeficiency Virus (HIV) is a member of the retroviridae family from lentivirus genus which primarily infects CD4+ T cells and also to lesser degree monocytes, macrophages, and dendritic cells causing progressive ... -
Structural and Related Studies on Mycobacterial RecA and LexA
(2018-06-18)Genetic material of bacteria is subject to damage due to multitudinous factors, both extrinsic and intrinsic in origin. Mechanisms for the maintenance of genomic integrity are thus essential for a bacterium to survive. ... -
Solution Structures and Dynamics of Conotoxins and Small MutS Related Domain from Helicobacter Pylori MutS2
(2018-06-08)The work presented in this thesis describes the determination of structures of peptides and proteins at atomic resolution. Nuclear Magnetic Resonance (NMR) spectroscopy was used as the principal method of investigation. ... -
(p)ppGpp and Stress Response : Decoding the Key Pathways by Small Molecule Analogues Biophysical Methods and Mass Spectrometry
(2018-06-08)Under hostile conditions, bacteria elicit stress response. Such stress response is regulated by a secondary messenger called (p)ppGpp. (p)ppGpp is involved in wide range of functions such as GTP homeostasis, biofilm formation ... -
Molecular Determinants of Mutant Phenotypes in the CcdAB Toxin -Antitoxin System
(2018-05-29)A major challenge in biology is to understand and predict the effect of mutations on protein structure, stability and function. Chapter 1 provides a general introduction on protein sequence-structure relationships and use ... -
Structural Studies on SeMV Chimeras and TSV : Insights into Capsid Assembly
(2018-05-23)Assembly of virus capsid protein (CP) into icosahedrally symmetric particles is an intriguing and elegant process. In most cases of virus assembly, a large number of identical protein subunits self-assemble to generate a ... -
Probing the Role of Highly Conserved Residues in Triosephosphate Isomerase : Biochemical & Structural Investigations
(2018-05-23)Conserved residues in protein are crucial for maintaining structure and function, either by direct involvement in chemistry or indirectly, by being essential for folding, stability and oligomerisation and are mostly clustered ... -
Effect of Ultrasound on Neuronal Network Communication
(2018-05-22)Low intensity and low frequency ultrasound has been shown to modulate ion channel currents, membrane capacitive currents, and as a result, neuronal activity. Ultrasound has been used as a non-invasive way to modulate ... -
Dynamics of Protein Kinases : Its Relationship to Functional Sites and States
(2018-05-22)A cell is a highly complex, ordered, and above all, a robust system. It copes with in-trennel and external uncertainties like heterogeneous stimuli, errors in processing and execution, and changes within and outside the ...