Browsing Biochemistry (BC) by Title
Now showing items 8-27 of 260
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Biochemical and Functional Characterization of Mycobacterium Tuberculosis Nucleoid-Associated Proteins H-NS and mIHF
(2018-08-11)Bacteria lack nucleus and any other membrane-bound organelles. Hence all the cellular components, including proteins, DNA, RNA and other components are located within the cytoplasm. The region of the cell which encompasses ... -
Biochemical Characterization Of An Acid-Adaptive Type III DNA Methyltransferase From Helicobacter Pylori 26695 And Its Biological Significance
(2014-12-15)Enzyme DNA methylation is an important biochemical process that imprints DNA with additional information. DNA methylation is catalyzed by S-adenosyl-L-methionine (AdoMet)-dependent methyltraferases (MTases). Prokaryotic ... -
Biochemical Characterization Of Heat Shock Protein 90 From Plasmodium Falciparum
(2013-06-07)Molecular chaperones are a group of proteins which maintain cellular homeostasis by assisting de novo protein folding and their refolding to native state after destabilization due to external stress. They are also known ... -
Biochemical Characterization Of Saccharomyces cerevisiae Mre11/Rad50/Xrs2 Using Telomeric DNA : A Role For The Endonucleolytic Activity Of Mre11 In Telomere Length Maintenance And Its Regulation By Rad50
(2009-05-15)Meiotic recombination is a prerequisite for exchange of genetic information in all Sexually reproducing organisms. This process is initiated by the formation of double stranded breaks (DSBs) in DNA followed by homology ... -
Biochemical characterization of UvrD helicase and its 1 interplay with DNA mismatch repair proteins in 2 Neisseria gonorrhoeae
UvrD helicase belongs to type I superfamily of helicases. It participates in Nucleotide Excision Repair (NER) and DNA Mismatch Repair (MMR). It also participates in the rolling circle replication. It has been shown that ... -
Biodegradation Of 1-Naphthoic Acid
(Indian Institute of Science, 2005-04-06)Polycyclic aromatic hydrocarbons (PAHs) are compounds containing carbon and hydrogen atoms of fused benzene rings in linear, angular or cluster arrangements. These compounds have a large (negative) resonance energy, resulting ... -
Characterisation Of DNA Llgase And Pairing Activities From A Partially Purified Fraction From Rat Testis
(Indian Institute of Science, 2005-04-06)Homologous genetic recombination is a central feature of meiosis in most sexually dividing organism. It leads to the establishment of new linkage relationships between genes and is crucial for the successful completion of ... -
Characterisation of Monoclonal Antibodies and Small Molecule Inhibitors as Hepatitis C Virus Entry Inhibitors
(2017-12-15)Hepatitis C virus (HCV) represents a global health threat. HCV is a blood-borne positive-strand RNA virus belonging to the Flaviviridae family that infects ~160 million people worldwide. About 70% of infected individuals ... -
Characterization Of HP1369-HP1370 From Helicobacter Pylori : A Novel ε Type N6 –Adenine Methyltransferase
(2013-07-18)Helicobacter pylori is one of the most genetically diverse bacterial species that successfully colonizes at least 50% of the world population. It has been associated with humans for thousands of years and most probably ... -
Characterization of mouse Apolipoprotein L9 and investigation of its cellular functions
This thesis is divided into 3 chapters. Chapter 1, titled General Introduction, is a chapter that familiarizes the reader with the general subject matter of the thesis so that the content of Chapters 2 and 3 can ... -
Characterization Of Structural And Non-structural Proteins Of Positive Sense, Single-stranded RNA Plant Viruses
(2015-07-08)In the present thesis, two positive sense single-stranded RNA viruses have been used as models to understand the structure and function of viral-encoded proteins. One of them, Pepper Vein Banding Virus (PVBV; genus Potyvirus; ... -
Characterization of Thymocyte Subpopulations and Mechanistic Insights during Various Modes of Thymic Atrophy
T lymphocytes along with B lymphocytes constitute the adaptive immune system, which is critical for host defence. The progenitors of T cells arise in the bone marrow and the sequential development of T cells occurs in the ... -
Chimeric Virus Like Particles as Nanocarriers for Antibody Delivery in Mammalian Cells & Role of Groundnut Bud Necrosis Virus NSs in Viral Life Cycle
(2018-08-02)Knowledge of the dissociation constants of the ionizable protons of weak acids in aqueous media is of fundamental importance in many areas of chemistry and biochemistry. The pKa value, or equilibrium dissociation constant, ... -
Cloning and Expression of a Diagnostic Antigen for Invasive Amoebiasis
(Indian Institute of Science, 2005-04-29)A crude extract of axenically grown amoebae was used as antigen in order to develop an AB microELISA for the detection and quantitation of E. histolytica-specific IgG antibodies. This ELISA was used to screen individual ... -
Comparative evaluation of retrograde regulatory proteins (Rtg1p, Rtg2p, and Rtg3p) of Saccharomyces cerevisiae and Pichia pastoris and identification of P. pastoris RtgXp as a novel regulator of multiple metabolic pathways
Our work demonstrates for the first time that PpRtgXp, a basic, helix-loop-helix (bHLH) protein, is a global regulator of carbon metabolism. PpRtgXp constitutively localizes inside the nucleus. Despite lacking the C-terminal ... -
A Comparative Study On The Sensitivity Of Cells Of Different Lineages To Plant Ribosome Inactivating Protein - Abrin
(2010-12-08)Proteins with selective toxicity have been investigated for use in many ways. One class of proteins, ribosome-inactivating proteins (RIPs), is found throughout the plant kingdom as well as in lower organisms like certain ... -
Computational and Experimental Approaches to Protein Design
Protein design is a rapidly maturing field, with the goal of designing proteins with customized structures and functions. A large number of protein structures are experimentally determined, which have facilitated an ... -
Conservation and divergence of the nucleotide excision repair pathway: Comparison of UvrB and UvrC proteins from Mycobacterium tuberculosis and Escherichia coli reveal significant differences in their functional properties
This thesis is presented in three chapters divided by topics. Chapter I will begin with an overview of literature regarding different types of DNA damage, DNA repair pathways in bacteria, properties of E. coli UvrABC ... -
Cytosolic Lysophosphatidic Acid Acyltransferase : Implications in Lipid Biosynthesis in Yeast, Plants and Human
(2018-02-11)Cytosolic LPA acyltransferase in yeast An isooctane tolerant strain of S. cerevisiae KK-12 was reported to have increased saturated fatty acid content (Miura et. al., 2000). Amongst the various genes upregulated on isooctane ...