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Investigation of RAG1 in lymphoid and brain cells: Understanding mechanism and regulation
In summary, we show that RAG1 expression is regulated through a novel miRNA (miR-
29c) mediated mechanism and establish that as an additional mode of RAG1 regulation.
Studies described in the thesis reveal that among the ...
Development and Characterization of a potent Tumor Necrosis Factor-alpha blocking agent
Tumor necrosis factor-α (TNFα), one of the major pro-inflammatory cytokines, plays a pivotal role in an effective inflammation process and immune response. TNFα interacts with two different receptors, TNFR1 and TNFR2, which ...
Conservation and divergence of the nucleotide excision repair pathway: Comparison of UvrB and UvrC proteins from Mycobacterium tuberculosis and Escherichia coli reveal significant differences in their functional properties
This thesis is presented in three chapters divided by topics. Chapter I will begin with an overview of literature regarding different types of DNA damage, DNA repair pathways in bacteria, properties of E. coli UvrABC ...
Production of taxol from Lasiodiplodia theobromae and biochemical characterization of its 10-deacetylbaccatin III-10-O-acetyltransferase enzyme and evaluation of anticancer activity of enzymatically synthesized baccatin III
Taxol (paclitaxel) originally identified from the inner bark of Taxus brevifolia, is a clinically approved anticancer drug for the treatment of various kinds of cancer, particularly ovarian cancer, breast cancer and Kaposi's ...
A role for the cohesin complex in subtelomeric gene silencing in Saccharomyces cerevisiae
Chromatin is a dynamic structure which reorganizes to support numerous chromosomal processes. Various histone and non-histone proteins are involved in chromatin organization. One such group of non-histone proteins, known ...
Mechanistic insights into the replication of Sesbania mosaic virus
The present thesis deals with the mechanism of replication of Sesbania mosaic virus (SeMV), which belongs to the genus sobemoviruses. Sobemoviruses are single stranded positive sense RNA viruses which infect both mono and ...
Understanding the Mechanism of Noncanonical Functions of RAGs in Lymphoid Cancer and Glioblastoma
Recombination activating genes (RAGs) composed of RAG1 and RAG2, are the endonuclease involved in V(D)J recombination, which is critical for adaptive immunity in mammals. Activation-induced cytidine deaminase (AID), expressed ...
A systems-level exploration of host responses across the disease spectrum of Tuberculosis and COVID-19
The host immune system orchestrates a defense mechanism against any invading pathogen with a combination of many mechanisms including antibody production, T-cell response, and memory response. The manner and extent of host ...
Establishment of human ectopic liver tissue in immunocompromised mice as a model for Hepatitis C Virus studies
Infection with hepatitis C virus (HCV) leads to an inflammatory condition which may progress to liver cirrhosis and in some cases, hepato-cellular carcinoma. Though, new drugs are being added to the list of HCV therapeutics, ...
Studies on the post-transcriptional regulation of genes of glutamate utilization pathway in Pichia pastoris and a role for Retrograde regulation protein 1
The methylotrophic yeast, Pichia pastoris can utilize compounds such as glucose, glycerol, methanol, acetate, amino acids, or oleic acid as the sole source of carbon. However, the regulation of synthesis of key enzymes of ...