Division of Biological Sciences: Recent submissions
Now showing items 161-180 of 1092
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Investigating the role and regulation of Polypyrimidine tract binding protein (PTB) in isoform switching during muscle development in Drosophila melanogaster
Muscles are an excellent system to dissect the development, physiology and evolution of cell heterogeneity and isoform switching. The vertebrate skeletal muscles are composed of heterogeneous muscle fibres. These fibres ... -
Elucidating the Role of Cyclic GMP in Diarrhoea and Intestinal Inflammation
Cyclic guanosine 3’,5’-monophosphate (cGMP) performs a wide range of functions in various cell types and tissues. The cellular levels of cGMP are maintained by the enzymatic conversion of guanosine 5’-triphosphate (GTP) ... -
A systems-level exploration of host responses across the disease spectrum of Tuberculosis and COVID-19
The host immune system orchestrates a defense mechanism against any invading pathogen with a combination of many mechanisms including antibody production, T-cell response, and memory response. The manner and extent of host ... -
Decoding Epigenetic Regulators in Cancer: Acetylation of HIF2A by Histone Acetyltransferase 1 (HAT1) is essential for executing hypoxia response in glioma
Gliomas are tumors of the central nervous system arising from glial cells. Based on the origin of the cell type, it can be astrocytoma, oligodendroglioma, and ependymoma. Astrocytoma is the most common type of glioma, ... -
Epithelial-to-mesenchymal transition and cellular cooperation in cancer progression: Novel roles for AMPK
Epithelial-mesenchymal transition (EMT) is a developmental program hijacked by cancer cells to facilitate metastasis —a multi-step process involving the spread of cancer cells from the primary tumor site and culminating ... -
Understanding the Long-term Impact and Mechanism of Action of Endosulfan, an Organochlorine Pesticide on Fertility, Development and Growth of Mice
Endosulfan (ES) is one of the major broad-spectrum organochlorine pesticides categorized as Class II (moderately hazardous) by WHO and Class I (highly acutely toxic) by US Environment Protection Agency. Although the use ... -
Structural insights into assembly and regulation of HigBA toxin-antitoxin system from Escherichia coli
In the last few decades, bacterial Toxin-antitoxin (TA) systems have been identified to play crucial roles in bacterial survival under stressful conditions and virulence. TA systems are pair of genetic elements where one ... -
Insights into the conformational stability & pharmacology of peptides through side chain and backbone modification
The first part of the work presented in the thesis deals with CH-pi interaction, which is a lesser-known non-covalent interaction in proteins. We identified several CH-pi donor-acceptor pairs involving amino acids side ... -
Homeostasis of cyclic-di-AMP in Mycobacterium smegmatis: Functional and structural contributions of c-di-AMP synthase (MsDisA) and hydrolase (MsPDE)
In bacteria, cyclic-di-nucleotide based second messengers regulate various physiological processes including the stress response. For the past decades, cyclic diadenosine monophosphate (c-di-AMP) has emerged as a crucial ... -
Transcription termination in Mycobacteria
Transcription termination is one of the key events of gene expression that decides the boundary of the gene. Termination either before the stop codon or transcriptional read-through into the downstream gene both have ... -
Understanding Nonhomologous DNA End Joining: Novel Insights Into Mechanism and Regulation
This study has provided new insights into the mechanistic details of NHEJ and showed that DNA sequence around the break site can be a factor in determining which proteins involved in NHEJ might be recruited with a higher ... -
Mechanistic insights into the role of miR‐6741‐3p in oral squamous cell carcinoma pathogenesis
Many functional studies and clinical analysis have linked miRNA dysregulation as a causative factor for tumor initiation and progression. Overexpression of oncogenic miRNAs and downregulation of tumor suppressor miRNAs are ... -
Mutational sensitivity of CcdB mutants in their native operonic context
Determining the precise effects of thousands of point mutants on protein structure, stability, and activity is a challenging task. Chapter 1 introduces how next-generation sequencing technologies exploit phenotypic screens ... -
Unravelling the functional role of Arf-like GTPases 14 and 15 in mammalian cells
Small G-proteins of Arf-like (Arl) GTPase subfamily are shown to regulate several cellular processes including intracellular trafficking, cytoskeletal organization, organelle biogenesis, cell adhesion and migration. Around ... -
Differential DNA methylation patterns in primary and recurrent glioblastomas : Protumorigenic roles of Tumor Endothelial Marker 8 (TEM8) and ASAP1 genes
Glioblastomas (GBMs) are clinically aggressive malignant brain tumors in adults with a median survival of 14 months on diagnosis. Therapeutic interventions such as debulking surgeries, chemotherapy, and ionizing radiation, ... -
Robustness of Neural Activity Dynamics in the Medial Entorhinal Cortex
Biological systems exhibit considerable heterogeneity in their constitutive components and encounter stochasticity across all scales of analysis. Therefore, central questions that span all biological systems are: (a) How ... -
Diversity and distribution of mixed-species foraging groups in the Lakshadweep Islands, India
Species interactions are known to shape biological communities. While antagonistic interactions like competition and predation are well known, cooperative interactions have received comparatively less attention. Mixed-species ... -
Mechanistic insights into protein stabilization and protein-protein interactions
Most amino acid substitutions in a protein either lead to partial loss of function or are near neutral. Several studies have shown the existence of second-site mutations that can rescue the defects caused by diverse loss ... -
Studies on the roles of rffG and rfbB encoding dTDP-glucose 4,6- dehydratase in Salmonella Typhimurium
Salmonellosis is a major health concern which causes significant morbidity and mortality worldwide. Infection with Salmonella Typhimurium leads to self-limiting diarrhea in healthy individuals and invasive disease in ... -
Structural, mechanistic and pharmacological studies of inhibitory neurotransmitter transporters
Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the central nervous system (CNS). The synaptic levels of GABA are controlled by the activity of GABA transporters (GATs) present in the presynaptic ...