Division of Biological Sciences: Recent submissions
Now showing items 141-160 of 1060
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Mutational sensitivity of CcdB mutants in their native operonic context
Determining the precise effects of thousands of point mutants on protein structure, stability, and activity is a challenging task. Chapter 1 introduces how next-generation sequencing technologies exploit phenotypic screens ... -
Unravelling the functional role of Arf-like GTPases 14 and 15 in mammalian cells
Small G-proteins of Arf-like (Arl) GTPase subfamily are shown to regulate several cellular processes including intracellular trafficking, cytoskeletal organization, organelle biogenesis, cell adhesion and migration. Around ... -
Differential DNA methylation patterns in primary and recurrent glioblastomas : Protumorigenic roles of Tumor Endothelial Marker 8 (TEM8) and ASAP1 genes
Glioblastomas (GBMs) are clinically aggressive malignant brain tumors in adults with a median survival of 14 months on diagnosis. Therapeutic interventions such as debulking surgeries, chemotherapy, and ionizing radiation, ... -
Robustness of Neural Activity Dynamics in the Medial Entorhinal Cortex
Biological systems exhibit considerable heterogeneity in their constitutive components and encounter stochasticity across all scales of analysis. Therefore, central questions that span all biological systems are: (a) How ... -
Diversity and distribution of mixed-species foraging groups in the Lakshadweep Islands, India
Species interactions are known to shape biological communities. While antagonistic interactions like competition and predation are well known, cooperative interactions have received comparatively less attention. Mixed-species ... -
Mechanistic insights into protein stabilization and protein-protein interactions
Most amino acid substitutions in a protein either lead to partial loss of function or are near neutral. Several studies have shown the existence of second-site mutations that can rescue the defects caused by diverse loss ... -
Studies on the roles of rffG and rfbB encoding dTDP-glucose 4,6- dehydratase in Salmonella Typhimurium
Salmonellosis is a major health concern which causes significant morbidity and mortality worldwide. Infection with Salmonella Typhimurium leads to self-limiting diarrhea in healthy individuals and invasive disease in ... -
Structural, mechanistic and pharmacological studies of inhibitory neurotransmitter transporters
Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the central nervous system (CNS). The synaptic levels of GABA are controlled by the activity of GABA transporters (GATs) present in the presynaptic ... -
Roles of Protein Acetylation in the Human Pathogen Helicobacter pylori
Helicobacter pylori is a gram-negative epsilon proteobacterium infecting half of the world population. H. pylori is a naturally competent bacterium with a huge repertoire of Restriction-Modification (RM) systems. The ... -
Immunological consequences of host signaling-regulated epigenetic modification(s) during mycobacterial pathogenesis
Introduction: Mycobacterium tuberculosis (Mtb), the principal etiological agent of pulmonary tuberculosis (TB), continues to co-evolve with the human population making itself one of the most potent infectious killer in the ... -
Characterization of the transsulfuration enzyme Cystathionine beta-synthase as a novel regulatory node in the sulfur metabolism of Mycobacterium tuberculosis
Methionine and cysteine metabolisms are important for the survival and pathogenesis of Mycobacterium tuberculosis (Mtb). The transsulfuration pathway converts methionine to cysteine and represents an important link between ... -
Regulation of Flagellar Motility in Giardia lamblia and Trichomonas vaginalis
Protozoan pathogens are responsible for infections that are highly prevalent, especially in developing and under-developed countries. Flagellar motility is exhibited by a variety of organisms ranging from bacteria to certain ... -
Role of calcium sensors in differential alignment of synaptic nanomachinery during neurotransmission
Some of the brain's most remarkable feats such as learning and memory, are thought to emerge from elementary properties of chemical synapses. The distinctive feature of these synapses is that the action potential in the ... -
Role of Mint family of Proteins in the nanoscale localization and real-time retention of surface Amyloid Precursor
Amyloid beta, a key determinant in the pathology of Alzheimer's disease (AD), is formed by the sequential proteolysis of Amyloid Precursor Protein (APP) by β-and γ-secretase. Evidence over the last few decades indicates ... -
The dual-targeting of Saccharomyces cerevisiae Pso2 into the nucleus and mitochondria is regulated by post-translational modifications: Implications for organelle-specific DNA repair
The repair of DNA interstrand crosslinks (ICLs) involves the concerted action of multiple DNA repair pathways. In eukaryotes, ICLs are sensed and repaired by replication-coupled or replication-independent mechanisms in ... -
Structural and Biophysical studies to evaluate the influence of oligomerization on biomolecular function
Oligomeric proteins are more common than monomers. This aspect of molecular evolution substantially contributes to functional diversity and the acquisition of features such as cooperativity and regulatory mechanisms. Not ... -
Studies on exosomal microRNAs and proteins associated with Hepatitis C and Dengue virus induced pathogenesis
Studies on the exosomal microRNAs and proteins associated with Hepatitis C and Dengue virus-induced pathogenesis. Hepatitis C virus and Dengue virus are the group of positive-strand RNA viruses belonging to the family ... -
Functional characterization of WD40-repeat protein, WDR8, in Zebrafish to gain insight into its role in Isolated Microspherophakia
WD40-repeat (WDR) proteins are a family of proteins that are characterized by widespread occurrence, low level of sequence conservation, common structural conformation (β propeller structure) and functional diversity. ... -
Structural and functional insights of biological macromolecules associated with infectious diseases – single particle cryo-electron microscopy based studies
In the global context, particularly in India, the two most important infectious diseases among many others are Tuberculosis (TB), an age-old disease and COVID-19, a new-age disease. This thesis focuses on some of the key ... -
Activin and TGF-β signaling: Differential role of a Serine/Threonine phosphate in the regulation of SMAD2 activity
Activins are the most prominent members of the Activin/Inhibin branch of the TGF-β superfamily. Similar to TGF-β, they play an essential role in regulating multiple physiological processes such as development, reproductive ...