Division of Biological Sciences: Recent submissions
Now showing items 121-140 of 1060
-
Insights into mRNA recruitment by eukaryotic initiation factor 4 during translation in Saccharomyces cerevisiae
Ribosomes are responsible for synthesizing proteins in the cells by using mRNA as the template. However, the ribosomes cannot perform this function without the help of other protein factors. Different factors are required ... -
Structural and biochemical studies on mycobacterial Uracil-DNA glycosylase (Ung) and MutT1, key proteins involved in maintaining the genomic integrity in Mycobacteria
Maintaining genomic integrity is indispensable for the survival and propagation of an organism. Failure to do so can cause mutations involving structural and functional aberrations, leading to severe diseases. The causative ... -
Stop codon readthrough of NNAT mRNA and its role in neuronal differentiation
Protein synthesis terminates at the first stop codon encountered by the ribosome. In stop codon readthrough, translation termination is suppressed, enabling the ribosomes to continue translation beyond the canonical stop ... -
Understanding the role of paraspeckle components NEAT1 and PSPC1 in HCV life cycle and pathogenesis
Hepatitis C virus (HCV) is an enveloped, small, icosahedral, positive-sense single-stranded RNA virus that belongs to the Hepacivirus genus and the Flaviviridae family. HCV causes acute and chronic hepatitis which can range ... -
Transcriptional regulation of a microRNA encoding gene MIR319C during leaf development in Arabidopsis thaliana
The evolutionarily conserved microRNA miR319 and its target transcription factors encoded by five CIN-TCPs (TCP2, 3, 4, 10 & 24) regulate leaf morphogenesis in Arabidopsis by triggering the division to differentiation ... -
Deciphering the mechanisms employed by Salmonella to mediate modulation of host endo-lysosomal machinery
Salmonella genus encompasses Gram-negative, rod-shaped, facultative anaerobic, non-sporulating, and predominantly motile enteric bacteria. It causes pathogenesis in a wide array of hosts, from cold-blooded animals to humans ... -
Post translational modification and DNA binding studies with two nucleoid associated proteins of Mycobacterium tuberculosis
Genome in prokaryotes is found in a small confinement called nucleoid. The nucleoid compaction is brought about by the combined action of topoisomerases and Nucleoid associated proteins (NAPs). Topoisomerases maintain ... -
Understanding the architecture of mitochondrial presequence translocase machinery and its implications in ALS progression
The current thesis describes the structural organization of the presequence translocase machinery and its functional association with ALS pathogenesis. The TIM23 complex is essential for the mitochondrial biogenesis of ... -
Investigating the role and regulation of Polypyrimidine tract binding protein (PTB) in isoform switching during muscle development in Drosophila melanogaster
Muscles are an excellent system to dissect the development, physiology and evolution of cell heterogeneity and isoform switching. The vertebrate skeletal muscles are composed of heterogeneous muscle fibres. These fibres ... -
Elucidating the Role of Cyclic GMP in Diarrhoea and Intestinal Inflammation
Cyclic guanosine 3’,5’-monophosphate (cGMP) performs a wide range of functions in various cell types and tissues. The cellular levels of cGMP are maintained by the enzymatic conversion of guanosine 5’-triphosphate (GTP) ... -
A systems-level exploration of host responses across the disease spectrum of Tuberculosis and COVID-19
The host immune system orchestrates a defense mechanism against any invading pathogen with a combination of many mechanisms including antibody production, T-cell response, and memory response. The manner and extent of host ... -
Decoding Epigenetic Regulators in Cancer: Acetylation of HIF2A by Histone Acetyltransferase 1 (HAT1) is essential for executing hypoxia response in glioma
Gliomas are tumors of the central nervous system arising from glial cells. Based on the origin of the cell type, it can be astrocytoma, oligodendroglioma, and ependymoma. Astrocytoma is the most common type of glioma, ... -
Epithelial-to-mesenchymal transition and cellular cooperation in cancer progression: Novel roles for AMPK
Epithelial-mesenchymal transition (EMT) is a developmental program hijacked by cancer cells to facilitate metastasis —a multi-step process involving the spread of cancer cells from the primary tumor site and culminating ... -
Understanding the Long-term Impact and Mechanism of Action of Endosulfan, an Organochlorine Pesticide on Fertility, Development and Growth of Mice
Endosulfan (ES) is one of the major broad-spectrum organochlorine pesticides categorized as Class II (moderately hazardous) by WHO and Class I (highly acutely toxic) by US Environment Protection Agency. Although the use ... -
Structural insights into assembly and regulation of HigBA toxin-antitoxin system from Escherichia coli
In the last few decades, bacterial Toxin-antitoxin (TA) systems have been identified to play crucial roles in bacterial survival under stressful conditions and virulence. TA systems are pair of genetic elements where one ... -
Insights into the conformational stability & pharmacology of peptides through side chain and backbone modification
The first part of the work presented in the thesis deals with CH-pi interaction, which is a lesser-known non-covalent interaction in proteins. We identified several CH-pi donor-acceptor pairs involving amino acids side ... -
Homeostasis of cyclic-di-AMP in Mycobacterium smegmatis: Functional and structural contributions of c-di-AMP synthase (MsDisA) and hydrolase (MsPDE)
In bacteria, cyclic-di-nucleotide based second messengers regulate various physiological processes including the stress response. For the past decades, cyclic diadenosine monophosphate (c-di-AMP) has emerged as a crucial ... -
Transcription termination in Mycobacteria
Transcription termination is one of the key events of gene expression that decides the boundary of the gene. Termination either before the stop codon or transcriptional read-through into the downstream gene both have ... -
Understanding Nonhomologous DNA End Joining: Novel Insights Into Mechanism and Regulation
This study has provided new insights into the mechanistic details of NHEJ and showed that DNA sequence around the break site can be a factor in determining which proteins involved in NHEJ might be recruited with a higher ... -
Mechanistic insights into the role of miR‐6741‐3p in oral squamous cell carcinoma pathogenesis
Many functional studies and clinical analysis have linked miRNA dysregulation as a causative factor for tumor initiation and progression. Overexpression of oncogenic miRNAs and downregulation of tumor suppressor miRNAs are ...