Molecular Biophysics Unit (MBU): Recent submissions
Now showing items 61-80 of 284
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Structural and functional studies on DNA damage inducible protein 1 (Ddi1) from protozoa
Structural and functional investigations on DNA-damage inducible protein1 (Ddi1) from Entamoeba histolytica, Trypanosoma cruzi and Toxoplasma gondii have been carried out. Ddi1 belongs to the ubiquitin receptor family of ... -
Computational Investigation of Heterogeneous Lateral Organisation in Biological Membrane
The study of lateral heterogeneity on membrane surface has come a long way from the fluid mosaic model. Simulations and experimental studies have observed non-random mixing of lipids and proteins in the membrane. In this ... -
Structural Studies on the Intracellular Steps that Govern the Staphylococcus aureus Quorum Sensing System.
Quorum sensing in bacteria has been extensively examined over the past two decades. These studies suggest that the molecular mechanism that governs quorum sensing incorporates niche-specific, environmentally sensitive ... -
Crystallographic and analytical ultracentrifugation studies of the E. coli acetohydroxy acid synthase I enzyme
This thesis ellucidates the mechanism of activation and regulation of AHAS I enzyme from Escherichia coli that was characterised using crystallographic and sedimentation velocity analytical ultracentrifugation studies. AHAS ... -
Small Heat Shock Proteins from Bacteria and a Bacteriophage
Proteins can unfold during heat and other types of stress and tend to aggregate with subsequent loss of function. This event is prevented by small Heat Shock Proteins (sHSPs) acting as molecular chaperones. sHSPs do not ... -
Scanning mutagenesis studies of HIV-1 Env immunogenicity and structure
Human Immunodeficiency Virus type 1 (HIV-1) is the etiological agent of Acquired Immunodeficiency Syndrome (AIDS). AIDS is a global pandemic with 36.7 million people living with HIV-1 all over the world and approximately ... -
Design, Synthesis, and Bioactivity of Thioamidated Macrocyclic Peptides
The work presented in this thesis describes the comprehensive study related to thioamidated macrocyclic peptides. We study the underexplored class of peptide bond isostere; thioamides in the context of small macrocyclic ... -
Studies on NMPylase and Valine Catabolism Enzymes from Mycobacterium
Post-translational modifications constitute an important arm of the regulatory mechanism in mammalian cells. Therefore, bacterial pathogens, which thrive by mimicking the host cell signaling, are proving to be difficult ... -
Molecular Characterization of Mannose Binding Lectins
Lectins are proteins of non-immunologic origin that bind to carbohydrates with high fidelity. They constitute a large class of multivalent recognition molecules that specifically interact with their cognate sugar moieties ... -
Structural Studies on Sesbania Mosaic Virus Protease Domain and Plasmodium Falciparum Hypoxanthine Quanine Phosphoribosyl Transferase and Triosephosphate Isomerase
X-ray crystallography is a powerful tool to obtain atomic resolution details on the three-dimensional structure of biological macromolecules. Crystallographic techniques find wide applications in understanding macromolecular ... -
Role of an RNA Polymerase Interacting Protein, MsRbpA, from Mycobacterium smegmatis in Phenotypic Tolerance to Rifampicin
Rifampicin is a non-competitive inhibitor of bacterial RNA polymerase (RNAP). The knowledge about the mechanism of action of this drug has emanated from the genetic and the biochemical studies carried out on Escherichia ... -
Synthesis and Conformational Analysis of Peptides Containing the Gamma Amino Acid Residue Gabapentin
Insertion of amino acid residues into all polypeptide backbones leads to an expanded repertoire of secondary structures. In the present thesis an attempt has been made to explore the conformational properties of the ... -
Ischemic Concentrations of Lactate Increase TREK1 Channel Activity and Expression
TREK1 is a two-pore domain leak potassium channel which contributes to resting membrane potential. TREK1-/- mice exhibit increased neuronal mortality after global ischemia. Neuroprotective agents like volatile anesthetics ... -
Structural and Functional Characterization of Enzymes of Proline Biosynthesis Pathway in Mycobacterium tuberculosis
Tuberculosis is the second largest killer disease on global scale after HIVAIDS. The rapid evolution of Mycobacterium tuberculosis into drug resistant strains and the toxicity of these drugs are the major constraints in ... -
Resource allocation during Flavivirus lifecycle
Single-stranded RNA virus infection cycle progresses by resource (viral RNA) allocation to segregated macromolecular complexes: host ribosomes (translation), viral RNA polymerase (replication) and viral structural proteins ... -
Understanding intramolecular signal transduction in regulated proteases of the High Temperature Requirement A family
Protein homeostasis in all organisms is a complex process involving regulatory mechanisms that govern protein synthesis, post-translational events and degradation. The protein degradation mechanism serves multiple functions ... -
Engineering Reverse Turns: Implications in Conformation, Stability and Bioactivity of Peptides and Proteins
The work presented in this thesis provides an overview of the potential role of N-methylation in inducing reverse turns. Backbone N-methylation is one of the common modifications designed by nature and exploited by humans ... -
Structural and related studies on Mycobacterium tuberculosis pantothenate kinase
Pantothenate kinase (PanK) is a ubiquitous and essential enzyme that catalyses the first step in the universal Coenzyme A (CoA) biosynthetic pathway. In this step pantothenate is converted to phosphopantothentate, which ... -
Development and application of sequence-based approaches for recognition and functional characterization of protein kinases
Protein kinases, the third most populous protein family, are a major class of enzymes that regulate a wide range of cellular processes by phosphorylating multiple cellular proteins. There are many conserved sequence motifs ...