Proteins play a central role in all biological processes. Despite a similar underlying composition, they exhibit immense diversity in structures and functions, a subject of great interest. The research presented in this ...

In the last few decades, bacterial Toxin-antitoxin (TA) systems have been identified to play crucial roles in bacterial survival under stressful conditions and virulence. TA systems are pair of genetic elements where one ...

Determining the precise effects of thousands of point mutants on protein structure, stability, and activity is a challenging task. Chapter 1 introduces how next-generation sequencing technologies exploit phenotypic screens ...

This thesis ellucidates the mechanism of activation and regulation of AHAS I enzyme from Escherichia coli that was characterised using crystallographic and sedimentation velocity analytical ultracentrifugation studies. AHAS ...

Bacterial type III toxin-antitoxin (TA) systems were first identified as abortive infection systems wherein a phage infection causes altruistic suicide of the bacterium to prevent bacteriophage spread among its clonal ...

Full text Embargo up to Sep 2026 Protein stability is critical to understanding protein function, folding, and interactions within living organisms. It plays a key role in protein engineering, where designing stable ...