Browsing Molecular Biophysics Unit (MBU) by Subject "Research Subject Categories::NATURAL SCIENCES::Biology::Cell and molecular biology"
Now showing items 1-20 of 25
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Achieving oral bioavailability of therapeutic macrocyclic peptides by bridging backbone chemistry with pharmacokinetics
Full text embargo up to Dec 11, 2026 Macrocyclic peptides are emerging as very potential candidates for drug development. Due to the advancement in display technologies, macrocycles can target undruggable proteins. These ... -
Characterizing the link between dynamics and function in proteins that violate the structure-function paradigm
The structure-function paradigm states that the three-dimensional structure of a protein is dictated by its amino acid sequence, which influences its function. However, this paradigm fails to explain the behaviour of ... -
Conformational and Dynamic Motifs in the Biomolecular Recognition of Glycan and Acetyl Functionalities
Biomolecular recognition is a fundamental principle underlying all cellular processes, driven by specific and tightly regulated interactions. This thesis explores the structural basis of recognition mechanisms in two ... -
Conformational Heterogeneity, Solution Structure and Autoregulatory Roles of the MazE9 Antitoxin from Mycobacterium tuberculosis
The work presented in this thesis endeavors to decode the details of the dynamics of MazE9 antitoxin of the Mycobacterium tuberculosis MazEF9 toxin-antitoxin system and the transcriptional autoregulation effected by it. ... -
Decoding determinants of selectivity in biomolecules
Molecular events demonstrate remarkable specificity amid complexity in its cellular environment. This phenomenon, termed biomolecular recognition, is fundamental to the organization and regulation of biological systems. ... -
Deep mutational scanning to enhance immunogenicity and map antibody responses to HIV-1
Vaccines have proven effective against numerous viral diseases, yet the development of a successful vaccine for Human Immunodeficiency Virus (HIV-1) remains elusive. A key immunogen for vaccine design is the surface ... -
Design of a thermostable pan-sarbecovirus vaccine
Coronaviruses (CoVs) are a global health threat due to their potential for widespread transmission including in humans. Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), the etiological agent responsible for ... -
Design of viral entry inhibitors and vaccines
Full text Embargo upto 12/12/2026 Human Immunodeficiency Virus type 1 (HIV-1) remains one of the most persistent global health threats, despite significant efforts in the development of vaccines and major strides in ... -
Development of quantitative RNA sequencing methods to understand RNA variant diversity.
The advent of next-generation sequencing (NGS) has accelerated biological research by providing the opportunity to connect genome level sequence information to function. One key application of NGS has been analysis of gene ... -
First thorough characterization of the structure, interactions and specificity of an archeal lectin with implication for TB infection
Decades long studies on plant lectins carried out in this laboratory have contributed substantially to glycobiology and helped in the initiation and development of macromolecular crystallography in India. Subsequently the ... -
Functional and structural studies of proteins involved in telomere maintenance
The linear ends of the eukaryotic chromosomes are protected by a specialized nucleoprotein complex known as telomere. Telomeres consist of telomere DNA and telomere DNA binding proteins (known as shelterin complex). The ... -
Homeostasis of cyclic-di-AMP in Mycobacterium smegmatis: Functional and structural contributions of c-di-AMP synthase (MsDisA) and hydrolase (MsPDE)
In bacteria, cyclic-di-nucleotide based second messengers regulate various physiological processes including the stress response. For the past decades, cyclic diadenosine monophosphate (c-di-AMP) has emerged as a crucial ... -
Immunogen design to enhance Influenza and SARS-CoV-2 vaccine efficacy
Influenza and SARS-CoV-2 are pleomorphic viruses that cause respiratory diseases in humans and present significant public health threats. Vaccination has been essential in controlling the spread of these infections and ... -
Integrative modeling of NS3 helicase function with molecular kinetics, molecular simulations and machine learning
The viral NS3 helicase is a crucial enzyme involved in the RNA replication of positive strand ssRNA viruses of the Flaviviridae family. The NS3 is an active motor that binds RNA and couples ATP hydrolysis to the mechanical ... -
Interaction of hnRNPK with RNA and characterization of its phase separation behavior
Heterogenous ribonucleoproteins (hnRNPs) are a class of proteins initially discovered to protect the nascent transcripts before they develop into primary mRNAs. They are involved in almost all the stages of mRNA metabolism, ... -
Molecular Characterization of Mannose Binding Lectins
Lectins are proteins of non-immunologic origin that bind to carbohydrates with high fidelity. They constitute a large class of multivalent recognition molecules that specifically interact with their cognate sugar moieties ... -
Mutational sensitivity of CcdB mutants in their native operonic context
Determining the precise effects of thousands of point mutants on protein structure, stability, and activity is a challenging task. Chapter 1 introduces how next-generation sequencing technologies exploit phenotypic screens ... -
Plasticity Paradigms: Navigating Protein Conformational Dynamics and Phenotypic Variability in Biological Systems
The complex dynamics and responses of biological systems to environmental cues are shaped by their inherent plasticity and adaptability. This biological plasticity, operational at multiple levels - ranging from the molecular ... -
Robustness of Neural Activity Dynamics in the Medial Entorhinal Cortex
Biological systems exhibit considerable heterogeneity in their constitutive components and encounter stochasticity across all scales of analysis. Therefore, central questions that span all biological systems are: (a) How ... -
Role of HCN Currents (Ih) in Epileptiform Activity in Subicular Neurons
Subicular burst firing neurons are established as primary instigators of seizure onset and propagation in Temporal Lobe Epilepsy (TLE), a form of focal epilepsy that accounts for approximately 60% of all epilepsy cases in ...