Browsing Molecular Biophysics Unit (MBU) by Title
Now showing items 98-117 of 284
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Mass Spectrometric Deconvolution of Libraries of Natural Peptide Toxins
(2018-03-24)This thesis deals with the analysis of natural peptide libraries using mass spectrometry. In the course of the study, both ribosomal and non-ribosomal classes of peptides have been investigated. Microheterogeneity, ... -
Mass Spectrometric Sequencing Of Acyclic And Cyclic Peptides
(2009-05-18)Elucidation of the primary structure of peptides and proteins de novo by mass spectrometry (MS) has become possible with the advent of tandem MS methods. The most widely used chemical method due to Edman (Edman & Begg, ... -
Mechanism Of Anticancer And Antimalarial Action Of A Modulator Of Heat Shock Proteins
(2007-11-16)This thesis entitled “Mechanism of Anticancer and Antimalarial Action of a Modulator of Heat Shock Proteins” describes the successful elucidation of the mechanism of anticancer and antimalarial action of 15-Deoxyspergualin ... -
Mechanism Of Interaction Of Escherichia Coli σ70 With Anti-Sigma Factors
(2009-08-03)In bacteria, the RNA polymerase (RNAP) consists of the following subunits: α2, β, β’, ω and σ. The core RNAP (α2ββ’ω) possesses the polymerising activity and it associates with one of the sigma factors to initiate transcription ... -
Mechanistic And Regulatory Aspects Of The Mycobacterium Tuberculosis Dephosphocoenzyme A Kinase
(2013-09-11)The current, grim world-TB scenario, with TB being the single largest infectious disease killer, warrants a more effective approach to tackle the deadly pathogen, Mycobacterium tuberculosis. The deadly synergy of this ... -
Mechanistic insights into protein stabilization and protein-protein interactions
Most amino acid substitutions in a protein either lead to partial loss of function or are near neutral. Several studies have shown the existence of second-site mutations that can rescue the defects caused by diverse loss ... -
Metabolic Adaptation For Utilization Of Short-Chain Fatty Acids In Salmonella Typhimurium : Structural And Functional Studies On 2-methylcitrate Synthase, Acetate And Propionate Kinases
(2013-08-22)Three-dimensional structures of proteins provide insights into the mechanisms of macromolecular assembly, enzyme catalysis and mode of activation, substrate-specificity, ligand-binding properties, stability and dynamical ... -
Modulation of Protein Stability and Function by Cysteine Mutations and Signal Peptides
(2018-06-23)Chapter 1gives a general introduction to the CXXC motif found in natural proteins. It then reviews the studies where disulphides were engineered in various proteins. The various strategies developed to engineer metal binding ... -
Molecular Characterization c-di-GMP Signalling In Mycobacterium Smegmatis
(2014-07-17)Bacterial stationary phase is an interesting biological system to study, as the organism undergoes several metabolic changes during this period and new molecules are generated to support its survival. The stationary phase ... -
Molecular Characterization of Bacillus Subtilis Oxidoreductases involved in the Bacilysin Synthesis
(2018-05-11)The biosynthetic pathway for the production of the dipeptide antibiotic bacilysin has been the subject of intense research over the past three decades. These studies revealed the role of multiple enzymes in the biosynthesis ... -
Molecular Characterization of Mannose Binding Lectins
Lectins are proteins of non-immunologic origin that bind to carbohydrates with high fidelity. They constitute a large class of multivalent recognition molecules that specifically interact with their cognate sugar moieties ... -
Molecular Determinants of Mutant Phenotypes in the CcdAB Toxin -Antitoxin System
(2018-05-29)A major challenge in biology is to understand and predict the effect of mutations on protein structure, stability and function. Chapter 1 provides a general introduction on protein sequence-structure relationships and use ... -
Multi-faceted modular interactions in proteins
All living systems are composed of plethora of biomolecules. There are various types of biomolecules such as proteins, DNA, RNA, carbohydrates and metabolites. All the reactions in a biological system involve interactions ... -
Mutational sensitivity of CcdB mutants in their native operonic context
Determining the precise effects of thousands of point mutants on protein structure, stability, and activity is a challenging task. Chapter 1 introduces how next-generation sequencing technologies exploit phenotypic screens ... -
Network Heterogeneities, Intrinsic Plasticity and Decorrelation in the Dentate Gyrus
A central premise for research in neuroscience is to mechanistically decipher the physiological roles of the brain in effectuating learning and memory. The tripartite framework that is at the core of learning and memory ... -
Neuronal complex bursts and network information transfer in the hippocampus are robust to biophysical heterogeneities
Biological entities must adopt mechanisms to override the impact of external perturbations to achieve stability and robustness. A crucial feature of biological systems is that they exhibit several forms of heterogeneities ... -
Newer Insights On Structure, Function And Regulation Of Dps Protein From Mycobacterium smegmatis
(2010-12-29)The first chapter will provide an introduction to the physiology, pathogenesis and biology of mycobacteria. Host-pathogen interactions, different modes of resistance of the bacteria, adaptations for survival under nutrient ... -
NMR Solution Structures of Human γC-Crystallin & the Intrinsically Disordered Viral Genome Linked Protein in the Free & Bound Form
(2018-02-14)This thesis describes the tertiary structures and dynamic studies of two protein systems. The first is human γC -crystallin protein, which is present in the nucleus of the human eye lens and the other is the plant viral ...