Browsing Molecular Biophysics Unit (MBU) by Title
Now showing items 42-61 of 281
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Designed β-Hairpin, β-Sheet And Mixed α-β Structures In Synthetic Peptides
(Indian Institute of Science, 2007-03-16)Synthetic construction of protein molecules has been widely pursued over the last two decades. A primary goal behind de novo protein design has been to build minimal systems by capturing the essential features of protein ... -
Determinants Of Globular Protein Stability And Temperature Sensitivity Inferred From Saturation Mutagenesis Of CcdB
(2011-02-25)The unique native structure is a basic requirement for normal functioning of most proteins. Many diseases stem from mutations in proteins that destabilize the protein structure thereby resulting in impairment or loss of ... -
Development and application of sequence-based approaches for recognition and functional characterization of protein kinases
Protein kinases, the third most populous protein family, are a major class of enzymes that regulate a wide range of cellular processes by phosphorylating multiple cellular proteins. There are many conserved sequence motifs ... -
Development And Applications Of Computational Methods To Aid Recognition Of Protein Functions And Interactions
(2011-10-03)Protein homology detection has played a central role in the understanding of evolution of protein structures, functions and interactions. Many of the developments in protein bioinformatics can be traced back to an initial ... -
Development of a Selective Cell-Permeable Protein Phosphatase 1 Inhibitor
(2018-07-10)Selective ‘super-specific’ inhibitors of Protein Phosphatase 1 (PP1) are not available. Several natural product toxins possessing marginal selectivity between PP1 and the closely related Protein Serine/Threonine Phosphatase ... -
Development of high throughput methodologies to isolate stabilized mutants and to identify interfacial residues
When eukaryotic or unstable proteins are overexpressed in bacteria, these often form insoluble aggregates called inclusion bodies (IB). Refolding the proteins from inclusion bodies can be challenging, resulting in low ... -
Development of quantitative RNA sequencing methods to understand RNA variant diversity.
The advent of next-generation sequencing (NGS) has accelerated biological research by providing the opportunity to connect genome level sequence information to function. One key application of NGS has been analysis of gene ... -
Differential Regulation Of tRNA1 Gly Genes In Bombyx Mori
(2012-03-30) -
Dissecting the C-DI-GMP Signaling Pathways : Tools and Tales
(2018-02-27)Evaluating aerodynamic noise from aircraft engines is a design stage process, so that it conform to regulations at airports. Aerodynamic noise is also a principal source of structural vibration and internal noise in ... -
The Dynamics of Iron in Miniferritins : A Structure-Function Connection
(2017-11-23)The DNA binding proteins under starvation (Dps) from M. smegmatis are cage-like structures which internalize iron and bind DNA. They provide resistance to the cells from free radical damage, and physically protect the DNA ... -
Dynamics of Protein Kinases : Its Relationship to Functional Sites and States
(2018-05-22)A cell is a highly complex, ordered, and above all, a robust system. It copes with in-trennel and external uncertainties like heterogeneous stimuli, errors in processing and execution, and changes within and outside the ... -
Effect of Ultrasound on Neuronal Network Communication
(2018-05-22)Low intensity and low frequency ultrasound has been shown to modulate ion channel currents, membrane capacitive currents, and as a result, neuronal activity. Ultrasound has been used as a non-invasive way to modulate ... -
Effects of Cyclic Permutation, Glycan Removal and Aspartate Mutagenesis on Conformation of HIV-1 Envelope
It has been decades since Human Immunodeficiency Virus-1 (HIV-1) was discovered. The virus has created havoc worldwide. According to the UNAIDS 2014 Gap report, 35million people worldwide are infected with HIV-1 and yet ... -
Elucidating the Role of MsRbpA in Rifampicin Tolerance and Transcription Regulation of Mycobacterium Smegmatis
(2018-04-12)RNA polymerase binding protein A (RbpA) was first discovered as a RNA polymerase binding protein from Streptomyces. coelicolor. It was shown to cause rifampicin tolerance to RNA polymerase in vitro and leads to basal level ... -
Emergence and Homeostasis of Functional Maps in Hippocampal Neurons
(2017-11-23)Systematic investigations through several experimental techniques have revealed that hippocampal pyramidal neurons express voltage gated ion channels (VGICs) with well-defined gradients along their dendritic arbor. These ... -
Endotoxin Peptide/Protein Interactions: Thermodynamic And Kinetic Analysis
(Indian Institute of Science, 2006-05-15)Endotoxin or Lipopolysaccharide (LPS) is the invariant structural component of gram negative bacterial outer membranes and is the chief causative factor of Sepsis or endotoxic shock. Sepsis is a syndrome that has very high ... -
Energetics Of Protein-Carbohydrate Recognition
(Indian Institute of Science, 2006-04-28)The work embodied in this thesis pertains to an attempt to understand better, the molecular basis of protein-carbohydrate recognition. For this purpose a systematic study was undertaken, not only of the energetics of ... -
Engineering Reverse Turns: Implications in Conformation, Stability and Bioactivity of Peptides and Proteins
The work presented in this thesis provides an overview of the potential role of N-methylation in inducing reverse turns. Backbone N-methylation is one of the common modifications designed by nature and exploited by humans ... -
Epileptiform Activity Induced Alterations In Ca2+ Dynamics And Network Physiology Of Hippocampal Neurons - In Vitro Studies
(2010-07-15)Epilepsy is characterized by the hyperexcitability of individual neurons and hyper synchronization of groups of neurons (networks). The acquired changes that take place at molecular, cellular and network levels are important ...