Browsing Molecular Biophysics Unit (MBU) by Title
Now showing items 35-54 of 284
-
Design and Characterization of HIV-1 ENV Derived Immunogens
(2018-06-21)The Human Immunodeficiency Virus (HIV) is a member of the retroviridae family from lentivirus genus which primarily infects CD4+ T cells and also to lesser degree monocytes, macrophages, and dendritic cells causing progressive ... -
Design And Isolation Of Temperature Sensitive Mutants Of Gal4 In Yeast And Drosophila
(2009-04-16)Genomic and proteomic investigations have yielded, and continue to produce, a large amount of information about genes and their protein products. In contrast, the evidence bearing on physiological roles of specific proteins ... -
Design and Stabilization of Stem Derived Immunogens from HA of Influenza A Viruses
(2018-07-19)Influenza virus belongs to the Orthomyxovirus family of viruses that causes respiratory infection in humans, leading to morbidity and mortality. The mature influenza A virion has an envelope that contains two major surface ... -
Design of Influenza Immunogens by Hemagglutinin (HA) Protein Minimization
(2018-01-31)Influenza virus is a pleiomorphic human pathogen which causes self-limiting respiratory illness lasting one-two weeks in most individuals. However, in immunologically compromised individuals, influenza infection may lead ... -
Design of soluble and particulate immunogens derived from the stem of Influenza hemagglutinin
Influenza is a highly contagious virus, belonging to the family Orthomyxoviridae that causes acute febrile respiratory illness which can be fatal in some cases. The highest risk groups for influenza viral infection ... -
Design, Synthesis, and Bioactivity of Thioamidated Macrocyclic Peptides
The work presented in this thesis describes the comprehensive study related to thioamidated macrocyclic peptides. We study the underexplored class of peptide bond isostere; thioamides in the context of small macrocyclic ... -
Designed Synthetic Peptides : Models For Studies Of Conformational Transitions And Aromatic Interactions
(2015-07-17)This thesis set out to explore the conformational properties of short designed peptide sequences, in which transitions between structural states may be anticipated. The use of conformationally constrained residues like ... -
Designed β-Hairpin, β-Sheet And Mixed α-β Structures In Synthetic Peptides
(Indian Institute of Science, 2007-03-16)Synthetic construction of protein molecules has been widely pursued over the last two decades. A primary goal behind de novo protein design has been to build minimal systems by capturing the essential features of protein ... -
Determinants Of Globular Protein Stability And Temperature Sensitivity Inferred From Saturation Mutagenesis Of CcdB
(2011-02-25)The unique native structure is a basic requirement for normal functioning of most proteins. Many diseases stem from mutations in proteins that destabilize the protein structure thereby resulting in impairment or loss of ... -
Determinants of protein stability and function inferred from saturation mutagenesis and next-generation sequencing
Proteins play a central role in all biological processes. Despite a similar underlying composition, they exhibit immense diversity in structures and functions, a subject of great interest. The research presented in this ... -
Development and application of sequence-based approaches for recognition and functional characterization of protein kinases
Protein kinases, the third most populous protein family, are a major class of enzymes that regulate a wide range of cellular processes by phosphorylating multiple cellular proteins. There are many conserved sequence motifs ... -
Development And Applications Of Computational Methods To Aid Recognition Of Protein Functions And Interactions
(2011-10-03)Protein homology detection has played a central role in the understanding of evolution of protein structures, functions and interactions. Many of the developments in protein bioinformatics can be traced back to an initial ... -
Development of a Selective Cell-Permeable Protein Phosphatase 1 Inhibitor
(2018-07-10)Selective ‘super-specific’ inhibitors of Protein Phosphatase 1 (PP1) are not available. Several natural product toxins possessing marginal selectivity between PP1 and the closely related Protein Serine/Threonine Phosphatase ... -
Development of high throughput methodologies to isolate stabilized mutants and to identify interfacial residues
When eukaryotic or unstable proteins are overexpressed in bacteria, these often form insoluble aggregates called inclusion bodies (IB). Refolding the proteins from inclusion bodies can be challenging, resulting in low ... -
Development of quantitative RNA sequencing methods to understand RNA variant diversity.
The advent of next-generation sequencing (NGS) has accelerated biological research by providing the opportunity to connect genome level sequence information to function. One key application of NGS has been analysis of gene ... -
Differential Regulation Of tRNA1 Gly Genes In Bombyx Mori
(2012-03-30) -
Dissecting the C-DI-GMP Signaling Pathways : Tools and Tales
(2018-02-27)Evaluating aerodynamic noise from aircraft engines is a design stage process, so that it conform to regulations at airports. Aerodynamic noise is also a principal source of structural vibration and internal noise in ... -
The Dynamics of Iron in Miniferritins : A Structure-Function Connection
(2017-11-23)The DNA binding proteins under starvation (Dps) from M. smegmatis are cage-like structures which internalize iron and bind DNA. They provide resistance to the cells from free radical damage, and physically protect the DNA ... -
Dynamics of Protein Kinases : Its Relationship to Functional Sites and States
(2018-05-22)A cell is a highly complex, ordered, and above all, a robust system. It copes with in-trennel and external uncertainties like heterogeneous stimuli, errors in processing and execution, and changes within and outside the ...