Browsing Molecular Biophysics Unit (MBU) by Title
Now showing items 269-288 of 393
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Structural and Mechanistic Studies of Gaba Transporter Isoform 1
The plasma membrane neurotransmitter transporter, GAT1, is responsible for the clearance of inhibitory neurotransmitter γ-aminobutyric acid (GABA) from the synaptic cleft. Targeting GABA reuptake by inhibiting GAT1, hence ... -
Structural And Mechanistic Studies On Receptor Protein Tyrosine Phosphatases From Drosophila Melanogaster
(2013-06-20)Protein Tyrosine Phosphatases (PTPs) initiate, modulate and terminate key cellular processes by dephosphorylating phosphotyrosine (pY) residues on signaling proteins. The coordinated action of PTPs with their cognate ... -
Structural and mechanistic studies on RNase J paralogs and associated multi-enzyme complexes in Staphylococcus aureus
The Staphylococcus aureus RNome, which includes both coding and non-coding RNAs, has a direct bearing on the virulence of this pathogen as it influences the ability to adapt to diverse environments within the host. Multiple ... -
Structural and mechanistic studies on Staphylococcal RNA degrading enzymes and multienzyme complexes
RNA degrading enzymes and multi-enzyme complexes govern a variety of cellular processes. The role of these enzymes and multi-enzyme assemblies has been suggested to govern gene expression levels in bacteria with modulations ... -
Structural and Related Studies on Mycobacterial Lectins
(2018-05-09)This thesis is concerned with the first ever X-ray crystallographic and complimentary solution studies on mycobacterial lectins. Lectins, described as multivalent carbohydrate binding proteins of non-immune origin, are ... -
Structural and Related Studies on Mycobacterial RecA and LexA
(2018-06-18)Genetic material of bacteria is subject to damage due to multitudinous factors, both extrinsic and intrinsic in origin. Mechanisms for the maintenance of genomic integrity are thus essential for a bacterium to survive. ... -
Structural and related studies on Mycobacterium tuberculosis pantothenate kinase
Pantothenate kinase (PanK) is a ubiquitous and essential enzyme that catalyses the first step in the universal Coenzyme A (CoA) biosynthetic pathway. In this step pantothenate is converted to phosphopantothentate, which ... -
Structural Characterization of Biological Macromolecules by Random Conical Tilt Pair Experiments
Structural biology deals with determining the structure of biological macromolecules especially proteins, DNA, RNA. The conformational changes in the structure of a macromolecule helps to decipher its function. This ... -
Structural Characterization Of Protein Folding Intermediates
(2013-07-25) -
Structural characterization of Staphylococcal γ-hemolysin in the presence of a lipid environment using single particle cryo-EM
γ-Hemolysin (γ-HL) is a quintessential bicomponent β-pore forming toxin (β-PFT) belonging to the arsenal of “superbug” Staphylococcus aureus. Here, we performed negative staining-TEM and single particle cryo-EM-based ... -
Structural Feature of Prokaryotic Promoters and their Role in Gene Expression
(2018-05-14)Transcription initiation is an important step in the process of gene regulation in prokaryotes. Promoters are stretches of DNA sequence that are present in the upstream region of transcription start sites (TSSs), where RNA ... -
Structural insights into assembly and regulation of HigBA toxin-antitoxin system from Escherichia coli
In the last few decades, bacterial Toxin-antitoxin (TA) systems have been identified to play crucial roles in bacterial survival under stressful conditions and virulence. TA systems are pair of genetic elements where one ... -
Structural insights into the organization and channel behavior of Pannexin isoforms
Pannexins are large-pore ion channels structurally related to Connexins and Innexins but remain as hemichannels to release cellular ATP upon activation. Pannexins comprise three isoforms, Pannexin1, 2, and 3, with diverse ... -
Structural Insights of Inter-domain Interactions in Multi-domain Proteins
Embargo up to August 11, 2026. Proteins are essential biomolecules whose diverse functions stem from their modular architecture built from independently folding units called domains. This thesis investigates the nature and ... -
Structural Investigations Of Sugar-Binding And Multivalency In Peanut Lectin
(2009-03-04)Starting with the structure analysis of ConA in the 70s, the crystal structures of hundreds of different lectins and their carbohydrate complexes have been determined. Lectins, multivalent carbohydrate-binding proteins ... -
Structural investigations on mycobacterial RecA
RecA protein plays a crucial role in homologous genetic recombination, DNA repair, and SOS induction. Homologous recombination involves genetic exchange between any two DNA molecules sharing extended regions with homologous ... -
Structural kinetic and mechanistic aspects of the carboxylic ionophore mediated transmembrane cation transport
Maintenance of ionic gradients across biological membranes has been established to be achieved mainly by an interplay of two mechanisms, namely, the carrier mechanism and the channel mechanism. In the carrier mechanism, ... -
Structural Studies Of E. Coli Thioredoxin And P. Falciparum Triosephosphate Isomerase By NMR And Computational Methods
(2014-05-21)To unravel the mysteries of complex biological processes carried out by biomolecules it is necessary to adopt a multifaceted approach, which involves employing a wide variety of tools both computational and experimental. ...