Browsing Molecular Biophysics Unit (MBU) by Title
Now showing items 261-280 of 284
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Substrate Specificity Determinants of Class III Nucleotide Cyclases
(2018-07-31)Cyclic AMP and cyclic GMP (cAMP and cGMP) are important second messengers in key signal-transduction pathways that mediate various physiological functions in bacteria and eukaryotes. Adenylyl Cyclases (ACs) and Guanylyl ... -
Subthreshold Conductances Regulate Theta-Frequency Local Field Potentials and Spike Phase
(2018-02-21)Local field potentials (LFPs), extracellular potentials that reflect localized electrical activity, have long been used as a window to understand the behavioural dependence and mechanistic aspects of brain physiology. A ... -
Suppression of epileptiform activity by lactate through HCA1 and GIRK channel activation in rat subicular pyramidal neurons
In the present study, we report a lactate-induced hyperpolarization and reduction of epileptiform activity in subicular weak burst firing and regular firing neurons using patch clamp electrophysiology. We showed the ... -
Synergistic Influence of Gene Architecture and Promoter Structure on Transcription in Plants : An in silico Analysis
Understanding the structural organisation of genome and insightful information of gene has helped in exploring the regulation and expression of the gene at a molecular and cellular level. In eukaryotes, the gene regulation ... -
Synthesis and Conformational Analysis of Peptides Containing the Gamma Amino Acid Residue Gabapentin
Insertion of amino acid residues into all polypeptide backbones leads to an expanded repertoire of secondary structures. In the present thesis an attempt has been made to explore the conformational properties of the ... -
A Systems Biology Approach towards Understanding Host Response and Pathogen Adaptation in Latent Tuberculosis Infection
(2018-01-04)Mycobacterium tuberculosis, the etiological agent of tuberculosis, has adapted with the host environment and evolved to survive in harsh conditions in the host. The pathogen has successfully evolved strategies not only to ... -
Targeting The CD4 Biniding Site In HIV-1 Immunogen Design
(2016-11-15)Over three decades have passed since the discovery of HIV-1, yet an AIDS vaccine remains elusive. The envelope glycoprotein of HIV-1 gp120, is the most exposed protein on the viral surface and thus serves as an important ... -
Temporal patterns of neural activity governing spatial representation in the hippocampus
Space is a fundamental substrate for animal interactions with the external world. The hippocampus, a region deep within the temporal lobe of mammalian brains, is a multisensory structure that has been heavily implicated ... -
Thermodynamic Characterization Of Wild Type And Mutants Of The E.coli Periplasmic Binding Proteins LBP, LIVBP, MBP And RBP
(2009-03-13)Native states of globular proteins typically show stabilization in the range of 5 to 15 kcal/mol with respect to their unfolded states. There has been a considerable progress in the area of protein stability and folding ... -
Theta-band Spectral Selectivity and Gamma-range Coincidence Detection in Spike Initiation Dynamics of Hippocampal Pyramidal Neurons
In the pursuit to understand information processing in the brain, a general question that is often posed is: What is the neuron encoding? This is addressed by assessing feature selectivity in sensory neurons, which informs ... -
Topology-based Sequence Design For Proteins Structures And Statistical Potentials Sensitive To Local Environments
(2013-01-17)Proteins, which regulate most of the biological activities, perform their functions through their unique three-dimensional structures. The folding process of this three dimensional structure from one dimensional sequence ... -
Understanding intramolecular signal transduction in regulated proteases of the High Temperature Requirement A family
Protein homeostasis in all organisms is a complex process involving regulatory mechanisms that govern protein synthesis, post-translational events and degradation. The protein degradation mechanism serves multiple functions ... -
Understanding protein structural excursions using residue networks and implications on biological function
The information required for a protein structure to fold into the native conformation is encoded in its sequence. Studying protein structures help to understand their biological function. Molecular interactions between ... -
Understanding structure, function, and dynamics in macromolecular complexes
In the scope of my thesis work, a concerted effort was undertaken to elucidate bi- ological functions through the lens of structural analysis, employing protein dy- namics. The role of protein dynamics in shaping and ... -
Understanding the interaction mechanism of bacterial virulence factors and effector proteins with host lipid membrane using single particle cryo-electron microscopy
Pathogenic bacteria employ an array of biological macromolecules and cellular structures that help in the initiation and progression of disease in host organism. These ‘virulence factors’ can either be secreted by the ... -
Understanding the Regulatory Steps that Govern the Activation of Mycobacterium Tuberculosis σK
(2018-07-28)A distinctive feature of host-pathogen interactions in the case of Mycobacterium tuberculosis is the asymptomatic latent phase of infection. The ability of the bacillus to survive for extended periods of time in the host ... -
Understanding the role of arginine clusters in β-hairpin antimicrobial peptides
The work described in this thesis describes the role of arginine clusters and the importance of their position in B-hairpin antimicrobial peptides. Here, we have tried to explore the role of guanidinium groups in the ...