Browsing Molecular Biophysics Unit (MBU) by Title
Now showing items 26-45 of 282
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Conformational Analysis Of Designed Alpha-Omega Hybrid Peptides
(2011-10-21)The insertion of ω- amino acid residues as guests into host α-peptide sequences permits expansion of the range of polypeptide secondary structures. The term ω- amino acid is used to refer to the entire family of residues ... -
Conformational Analysis of Designed and Natural Peptides : Studies of Aromatic/Aromatic and Aromatic/Proline Interactions by NMR
(2018-04-05)This thesis describes NMR studies which probe weak interactions between amino acid side chains in folded peptide structures. Aromatic/aromatic interactions between facing phenylalanine residues have been probed in antiparallel ... -
Conformational Heterogeneity, Solution Structure and Autoregulatory Roles of the MazE9 Antitoxin from Mycobacterium tuberculosis
The work presented in this thesis endeavors to decode the details of the dynamics of MazE9 antitoxin of the Mycobacterium tuberculosis MazEF9 toxin-antitoxin system and the transcriptional autoregulation effected by it. ... -
Crystal Structure Of Jacalin At 3.0A Resolution
(2012-11-15) -
Crystallization and Preliminary Structural Studies on Mycobacterium tuberculosis RuvX
The deadly pathogen Mycobacterium tuberculosis has been characterized for its virulence in immense detail. Regardless of this, the capability of M. tuberculosis to combat extreme stress conditions inside the host cell for ... -
Crystallographic and analytical ultracentrifugation studies of the E. coli acetohydroxy acid synthase I enzyme
This thesis ellucidates the mechanism of activation and regulation of AHAS I enzyme from Escherichia coli that was characterised using crystallographic and sedimentation velocity analytical ultracentrifugation studies. AHAS ... -
Deciphering Structure-Function Relationships in a Two-Subunit-Type GMP Synthetase by Solution NMR Spectroscopy
(2018-04-24)The guanosine monophosphate synthetase (GMPS) is a class I glutamine amidotransferase, involved in the de-novo purine nucleotide biosynthesis. The enzyme catalyzes the biochemical transformation of xantosine (XMP) into ... -
Deep mutational scanning to enhance immunogenicity and map antibody responses to HIV-1
Vaccines have proven effective against numerous viral diseases, yet the development of a successful vaccine for Human Immunodeficiency Virus (HIV-1) remains elusive. A key immunogen for vaccine design is the surface envelope ... -
Design and Characterization of HIV-1 ENV Derived Immunogens
(2018-06-21)The Human Immunodeficiency Virus (HIV) is a member of the retroviridae family from lentivirus genus which primarily infects CD4+ T cells and also to lesser degree monocytes, macrophages, and dendritic cells causing progressive ... -
Design And Isolation Of Temperature Sensitive Mutants Of Gal4 In Yeast And Drosophila
(2009-04-16)Genomic and proteomic investigations have yielded, and continue to produce, a large amount of information about genes and their protein products. In contrast, the evidence bearing on physiological roles of specific proteins ... -
Design and Stabilization of Stem Derived Immunogens from HA of Influenza A Viruses
(2018-07-19)Influenza virus belongs to the Orthomyxovirus family of viruses that causes respiratory infection in humans, leading to morbidity and mortality. The mature influenza A virion has an envelope that contains two major surface ... -
Design of Influenza Immunogens by Hemagglutinin (HA) Protein Minimization
(2018-01-31)Influenza virus is a pleiomorphic human pathogen which causes self-limiting respiratory illness lasting one-two weeks in most individuals. However, in immunologically compromised individuals, influenza infection may lead ... -
Design of soluble and particulate immunogens derived from the stem of Influenza hemagglutinin
Influenza is a highly contagious virus, belonging to the family Orthomyxoviridae that causes acute febrile respiratory illness which can be fatal in some cases. The highest risk groups for influenza viral infection ... -
Design, Synthesis, and Bioactivity of Thioamidated Macrocyclic Peptides
The work presented in this thesis describes the comprehensive study related to thioamidated macrocyclic peptides. We study the underexplored class of peptide bond isostere; thioamides in the context of small macrocyclic ... -
Designed Synthetic Peptides : Models For Studies Of Conformational Transitions And Aromatic Interactions
(2015-07-17)This thesis set out to explore the conformational properties of short designed peptide sequences, in which transitions between structural states may be anticipated. The use of conformationally constrained residues like ... -
Designed β-Hairpin, β-Sheet And Mixed α-β Structures In Synthetic Peptides
(Indian Institute of Science, 2007-03-16)Synthetic construction of protein molecules has been widely pursued over the last two decades. A primary goal behind de novo protein design has been to build minimal systems by capturing the essential features of protein ... -
Determinants Of Globular Protein Stability And Temperature Sensitivity Inferred From Saturation Mutagenesis Of CcdB
(2011-02-25)The unique native structure is a basic requirement for normal functioning of most proteins. Many diseases stem from mutations in proteins that destabilize the protein structure thereby resulting in impairment or loss of ... -
Development and application of sequence-based approaches for recognition and functional characterization of protein kinases
Protein kinases, the third most populous protein family, are a major class of enzymes that regulate a wide range of cellular processes by phosphorylating multiple cellular proteins. There are many conserved sequence motifs ...