Browsing Molecular Biophysics Unit (MBU) by Title
Now showing items 192-211 of 393
-
Protein NMR Studies of E. Coli IlvN and the Protease-VPg Polyprotein from Sesbania Mosaic Virus
(2018-04-03)Acetohydroxyacid synthase is a multisubunit enzyme that catalyses the first committed step in the biosynthesis of the branched chain amino acids viz., valine, leucine and isoleucine. In order to understand the structural ... -
Protein Structure and Mutant Phenotype Prediction from Analysis of Mutant Libraries by Deep Sequencing
Proteins play a central role in all the biological processes. The immense diversity in protein structures and functions despite similar underlying composition is intriguing. The work presented in this thesis aims to provide ... -
Protein-DNA Graphs And Interaction Energy Based Protein Structure Networks
(2013-01-29)Proteins orchestrate a number of cellular processes either alone or in concert with other biomolecules like nucleic acids, carbohydrates, and lipids. They exhibit an intrinsic ability to fold de novo to their functional ... -
Protein-small molecule interactions: Structural insights and applications in computational drug discovery
Deviation from normal healthy conditions, termed as disease, can often be triggered due to the malfunctioning of proteins. Modulating the functions of proteins by administering therapeutic agents (drugs) may alleviate the ... -
Pseudo-periodic oscillatory dependence of rat brain voltage gated sodium channel properties on prior depolarization, studied using whole cell patch clamp, wavelet analysis and single neuron simulation
Neuronal excitability is mediated by ion-selective transmembrane ion channels, through which ions are transported in response to changes in membrane potential. In particular, the rising phase of the action potential is ... -
Rational Elicitation of Cold Sensitive Phenotypes
Conditional gene expression and conditional mutants provide a means to modulate the expression of specific genes and to control the activity of their protein products in vivo, so as to be able to study their effects on the ... -
Repetitive sequences, unusual DNA structures and chromatin organisation
Since the discovery of DNA and the proposal of the double helix structure, we have come a long way in understanding the molecular basis of heredity. With the ability to synthesize any DNA sequence of interest and improved ... -
Resource allocation during Flavivirus lifecycle
Single-stranded RNA virus infection cycle progresses by resource (viral RNA) allocation to segregated macromolecular complexes: host ribosomes (translation), viral RNA polymerase (replication) and viral structural proteins ... -
Robustness of Neural Activity Dynamics in the Medial Entorhinal Cortex
Biological systems exhibit considerable heterogeneity in their constitutive components and encounter stochasticity across all scales of analysis. Therefore, central questions that span all biological systems are: (a) How ... -
Role of an RNA Polymerase Interacting Protein, MsRbpA, from Mycobacterium smegmatis in Phenotypic Tolerance to Rifampicin
Rifampicin is a non-competitive inhibitor of bacterial RNA polymerase (RNAP). The knowledge about the mechanism of action of this drug has emanated from the genetic and the biochemical studies carried out on Escherichia ... -
Role of autophagy in reducing peripheral neuropathy and diabetic peripheral neuropathy
Peripheral neuropathy occurs when there is damage to the central or peripheral nervous system due to injury, disease, diabetes, or drug use. Endoplasmic reticulum (ER) stress is a distinct feature observed in neuropathic ... -
The Role of DNA Structural Features of Eukaryotic Promoter Sequences in Transcription Regulation
(2018-09-04)Understanding the molecular structure of DNA was considered as greatest achievement in modern biology. It helped in understanding fundamental cellular processes such as replication of DNA, nature of the ... -
Role of Glycosylation and Oligomerization on the Stability of Soybean Agglutinin
Carbohydrates are vital to life. In their naive form, they serve as a primary energy source for supporting life. However, in most cases carbohydrates do not exist as simple sugars in nature. Instead they occur as more ... -
Role of HCN Currents (Ih) in Epileptiform Activity in Subicular Neurons
Subicular burst firing neurons are established as primary instigators of seizure onset and propagation in Temporal Lobe Epilepsy (TLE), a form of focal epilepsy that accounts for approximately 60% of all epilepsy cases in ... -
Role of Lactate and TREK1 Channels in Neuroprotection during Cerebral Ischemia – in Vitro Study in Rat Hippocampus
(2017-12-16)Cerebral ischemia is a highly debilitating condition where shortage of oxygen and glucose leads to profuse cell death. Insufficient blood supply to the brain leads to cerebral ischemia and increase in extracellular lactate ... -
Role of RNA Polymerase ω Subunit in Metabolism and Stress Response
RNA polymerase (RNAP) is the key enzyme in transcription and it is a multi-subunit enzyme made up of alpha, beta, beta prime and omega subunit in the stoichiometry of α2ββω. Except for the smallest subunit ω (rpoZ), all ...