Browsing Molecular Biophysics Unit (MBU) by Title
Now showing items 128-147 of 393
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Hydration, mobility and action of proteins : an x-ray study involving hen-egg-white lysozyme and human hemoglobin
Hydration and mobility of proteins have received considerable attention. It is also recognized that the two are related. A long-range protein crystallographic program in this laboratory is concerned with protein hydration ... -
Identification, characterization, structure, and assembly of type III toxin-antitoxin systems from Escherichia coli
Bacteria adopt several defense strategies to enable their survival against the environmental threats they encounter from time to time. Toxin-antitoxin (TA) systems are being understood as a key bacterial defense mechanism ... -
Immunogen design to enhance Influenza and SARS-CoV-2 vaccine efficacy
Influenza and SARS-CoV-2 are pleomorphic viruses that cause respiratory diseases in humans and present significant public health threats. Vaccination has been essential in controlling the spread of these infections and ... -
In Silico Perspectives on RNA Structures Modulating Viral Gene Expression and Mechanics of tRNA Transport
(2018-07-19)The repertoire of cellular functions mediated by Ribonucleic acid (RNA) molecules have expanded considerably during the last two decades. The role played by RNA in controlling and regulating gene expression in ... -
Influence of the substrate specificity of Mycobacterium tuberculosis ClpX on the transcriptional profile
Cellular homeostasis in bacteria is maintained by diverse molecular machines. These include several one-component systems (OCS), two- component systems (TCS), chaperone proteins, proteases, proteolytic complexes and ... -
Insights into the conformational stability & pharmacology of peptides through side chain and backbone modification
The first part of the work presented in the thesis deals with CH-pi interaction, which is a lesser-known non-covalent interaction in proteins. We identified several CH-pi donor-acceptor pairs involving amino acids side ... -
Insights Into The Mechanistic Details Of The M.Tuberculosis Pantothenate Kinase : The Key Regulatory Enzyme Of CoA Biosynthesis
(2013-04-26)Tuberculosis (TB), caused by Mycobacterium tuberculosis, has long been the scourge of humanity, claiming millions of lives. It is the most devastating infectious disease of the world in terms of mortality as well as morbidity ... -
Integration of sequences, structures, dynamics to study functional divergence in homologous proteins and their assemblies
Functions of proteins are governed by their sequences, structures, dynamics and assembly. Modulation of these factors during evolution renders functional divergence in related proteins that have originated from the common ... -
Integrative modeling of NS3 helicase function with molecular kinetics, molecular simulations and machine learning
The viral NS3 helicase is a crucial enzyme involved in the RNA replication of positive strand ssRNA viruses of the Flaviviridae family. The NS3 is an active motor that binds RNA and couples ATP hydrolysis to the mechanical ... -
Integrative multiscale modeling and simulations of biomolecules with experimentally testable predictions
This thesis delves into four separate research works related to Integrative multiscale modeling and simulations of biomolecules. The first work deals with the puzzling case of the Pleckstrin Homology Domain (PHD), which ... -
Interaction Of Chaperone SecB With Protein Substrates: A Biophysical Study
(Indian Institute of Science, 2006-10-17)In the cell, as in in vitro, the final conformation of a protein is determined by it's amino acid sequence (1). Some isolated proteins can be denatured and refolded in vitro in absence of extrinsic factors. However, in ... -
Interaction of hnRNPK with RNA and characterization of its phase separation behavior
Heterogenous ribonucleoproteins (hnRNPs) are a class of proteins initially discovered to protect the nascent transcripts before they develop into primary mRNAs. They are involved in almost all the stages of mRNA metabolism, ... -
Intracellular Calcium Dynamics In Dendrites Of Hippocampal Neurons Rendered Epileptic And In Processes Of Astrocytes Following Glutamate Pretreatment
(2009-04-03)The fundamental attribute of neurons is their cellular electrical excitability, which is based on the expression of a plethora of ligand- and voltage-gated membrane channels that give rise to prominent membrane currents ... -
Intrinsic Versus Induced Variations In DNA Structure
(2011-07-18)The binding of different proteins involved in processes such as transcription, replication and chromatin compaction to regions of the genome is regulated by the structure of DNA. Thus, DNA structure acts as the crucial ... -
Ischemic Concentrations of Lactate Increase TREK1 Channel Activity and Expression
TREK1 is a two-pore domain leak potassium channel which contributes to resting membrane potential. TREK1-/- mice exhibit increased neuronal mortality after global ischemia. Neuroprotective agents like volatile anesthetics ... -
Kinetic and thermodynamic study of a non-inactivating K+ current in a gonadotroph cell-line
aT3-1 is a gonadotroph cell line derived from mice by oncogenic transformation of the pituitary, using simian virus, and secretes a subunit of follicle-stimulating hormone in response to hypothalamic peptide gonadotropin-releasing ... -
Legume lectin carbohydrate regognition : clonning of peanut( arachits hypogea) and winged bean (psophocarpus tetragono;obus) agglutinins and reswsign of carbohydrate specificity of PNA
Recognition of cell-surface carbohydrates by lectins has wide implications in important biological processes such as protein targeting to cellular compartments, homing of lymphocytes, host-pathogen interactions, and ... -
Lithium Exposure Induced Changes At Glutamatergic Synapses In Hippocampal Neurons- Insights From In Vitro Electrophysiology And Imaging Studies
(2016-11-15)Lithium is a drug used to treat mood disorders and also has many side effects, including central nervous system (CNS) complications (such as cognitive dulling), associated with its use. The mechanism of its action still ... -
Mass Spectrometric Deconvolution of Libraries of Natural Peptide Toxins
(2018-03-24)This thesis deals with the analysis of natural peptide libraries using mass spectrometry. In the course of the study, both ribosomal and non-ribosomal classes of peptides have been investigated. Microheterogeneity, ...