• Login
    View Item 
    •   etd@IISc
    • Division of Chemical Sciences
    • Inorganic and Physical Chemistry (IPC)
    • View Item
    •   etd@IISc
    • Division of Chemical Sciences
    • Inorganic and Physical Chemistry (IPC)
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Turning an Antioxidant into a Prooxidant: Light-induced Mitochondrial Apoptosis in Mammalian Cells by an Ergothioneine-based Neutral Small Molecule

    Thumbnail
    View/Open
    Thesis full text (18.98Mb)
    Author
    Pundlik, Raul Kusaji
    Metadata
    Show full item record
    Abstract
    Mitochondria-targeted compounds gained significant interest due to their applications in fundamental research, diagnostics, and therapeutics. In this work, we present a detailed investigation of the ergothioneine-based mitochondria-targeted neutral molecule, which is an antioxidant in the dark and turns prooxidant in light inside mammalian cells. This molecule was designed using a fragment-based approach for simultaneous mitochondrial imaging and targeted antioxidant activity. However, it forms a charge-separated state within 10 picoseconds under visible light, leading to triplet-state formation upon charge recombination. Mitochondrial biomolecules first reduce this redox-active triplet state to its one-electron reduced form, which reduces molecular oxygen via one-electron transfer to form superoxide radicals selectively inside mitochondria. This leads to mitochondrial lipid peroxidation, increased mitochondrial membrane permeability transition pore formation, mitochondrial depolarization, and eventually, apoptotic cell death.
    URI
    https://etd.iisc.ac.in/handle/2005/6981
    Collections
    • Inorganic and Physical Chemistry (IPC) [303]

    etd@IISc is a joint service of SERC & J R D Tata Memorial (JRDTML) Library || Powered by DSpace software || DuraSpace
    Contact Us | Send Feedback | Thesis Templates
    Theme by 
    Atmire NV
     

     

    Browse

    All of etd@IIScCommunities & CollectionsTitlesAuthorsAdvisorsSubjectsBy Thesis Submission DateThis CollectionTitlesAuthorsAdvisorsSubjectsBy Thesis Submission Date

    My Account

    LoginRegister

    etd@IISc is a joint service of SERC & J R D Tata Memorial (JRDTML) Library || Powered by DSpace software || DuraSpace
    Contact Us | Send Feedback | Thesis Templates
    Theme by 
    Atmire NV