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dc.contributor.advisorJayaprakash, Balaji
dc.contributor.advisorMarathe, Swananda
dc.contributor.authorNandi, Arnab
dc.date.accessioned2024-04-15T10:13:46Z
dc.date.available2024-04-15T10:13:46Z
dc.date.submitted2024
dc.identifier.urihttps://etd.iisc.ac.in/handle/2005/6486
dc.description.abstractThis thesis investigates the enduring effects of early-life stress, specifically maternal separation, on astroglial population and the subsequent behavioral outcomes in adult mice. While the persistent impact of early-life stress on physiology and behavior is well-documented, the role of astrocytes in mediating these effects remains largely unexplored. Employing a comprehensive approach, we investigate immediate post-stress cell proliferation, cellular composition and conduct a detailed morphometric analysis of hippocampal astrocytes during late adulthood. Our study revealed a reduction in cell proliferation and subsequent astrocyte decrease at six months, linked to depressive-like behavior in maternally stressed mice. Our findings uncover astrocytic hypertrophy persisting in adulthood post maternal separation, suggesting a lasting effect on astroglia. In tandem with the cellular assessments, this study integrates behavioral analyses utilizing the forced swim test and tail suspension test, shedding light on the depressive-like behavior associated with early-life stress. In addition to the experimental investigations, this thesis introduces ‘DBscorer’ and ‘tracKit’, two open-source MATLAB software with an intuitively designed Graphical User Interface (GUI) for precise measurement in behavioral experiments. Addressing the computational complexities often faced by behavioral laboratories, this two software provides a user-friendly and accurate solution, saving valuable time and resources for researchers. This research advances our understanding of the enduring impact of early-life stress on cellular proliferation, astroglial changes and provides a practical tool for the broader neuroscience community, facilitating precise and efficient analysis for various behavioral assessments in laboratory settings.en_US
dc.description.sponsorshipCSIRen_US
dc.language.isoen_USen_US
dc.relation.ispartofseries;ET00495
dc.rightsI grant Indian Institute of Science the right to archive and to make available my thesis or dissertation in whole or in part in all forms of media, now hereafter known. I retain all proprietary rights, such as patent rights. I also retain the right to use in future works (such as articles or books) all or part of this thesis or dissertationen_US
dc.subjectMouse behavioren_US
dc.subjectMATLAB softwareen_US
dc.subjectImage analysisen_US
dc.subjectAstrocyte developmenten_US
dc.subjectGliaen_US
dc.subjectEarly life stressen_US
dc.subjectMaternal separationen_US
dc.subjectDepressionen_US
dc.subject.classificationResearch Subject Categories::NATURAL SCIENCES::Biology::Other biologyen_US
dc.titleAstroglial development as the basis for the effects of early life stress on adult depressive-like behavior​en_US
dc.typeThesisen_US
dc.degree.namePhDen_US
dc.degree.levelDoctoralen_US
dc.degree.grantorIndian Institute of Scienceen_US
dc.degree.disciplineFaculty of Scienceen_US


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