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dc.contributor.advisorGopal, B
dc.contributor.authorChintamani, Joshi Anuja
dc.date.accessioned2021-10-22T09:14:06Z
dc.date.available2021-10-22T09:14:06Z
dc.date.submitted2018
dc.identifier.urihttps://etd.iisc.ac.in/handle/2005/5466
dc.description.abstractCellular homeostasis in bacteria is maintained by diverse molecular machines. These include several one-component systems (OCS), two- component systems (TCS), chaperone proteins, proteases, proteolytic complexes and transcription factors that regulate protein synthesis and recycling. Protein and oligonucleotide recycling provides a route to clear the cell of aggregated and non-functional bio-molecules thereby mitigating metabolic stress and ensuring bacterial survival. Protein recycling machines in bacteria have been examined in different contexts. These include proteases that function in isolation and chaperone assisted proteases for degradation of specific bio-molecules. The focus of the work reported in this thesis was to understand the mechanism by which proteolytic assemblies influence intracellular signal transduction. This aspect is of particular relevance for the human pathogen Mycobacterium tuberculosis due to its growth features, ability to endure diverse micro-environments inside the host and a pronounced latent phase prior to onset of disease. M. tuberculosis has several proteases that include FtsH (involved in cell division), Clp proteases- ClpX, ClpC1, ClpP1 and ClpP2 (involved in the maintenance of cellular homeostasis) and the proteasome. Unlike Escherichia coli and Bacillus subtilis, there are no homologues of Lon and HslUV proteases in M. tuberculosis. Indeed, all the Clp proteases in M. tuberculosis are essential. The work described in this thesis reveals the basis by which substrate selectivity is enforced by these chaperone-proteases with direct and indirect repercussions on bacterial adaptation and cellular changes. These studies suggest a link between protein recycling mechanisms and the transcription process suggesting feed-forward and feed-back loops that ensure cellular homeostasis and adaptation to diverse stressesen_US
dc.language.isoen_USen_US
dc.relation.ispartofseries;G29291
dc.rightsI grant Indian Institute of Science the right to archive and to make available my thesis or dissertation in whole or in part in all forms of media, now hereafter known. I retain all proprietary rights, such as patent rights. I also retain the right to use in future works (such as articles or books) all or part of this thesis or dissertationen_US
dc.subjectCellular homeostasisen_US
dc.subjectproteolytic assembliesen_US
dc.subjectintracellular signal transductionen_US
dc.subjectMycobacterium tuberculosisen_US
dc.subject.classificationResearch Subject Categories::NATURAL SCIENCES::Biologyen_US
dc.titleInfluence of the substrate specificity of Mycobacterium tuberculosis ClpX on the transcriptional profileen_US
dc.typeThesisen_US
dc.degree.namePhDen_US
dc.degree.levelDoctoralen_US
dc.degree.grantorIndian Institute of Scienceen_US
dc.degree.disciplineFaculty of Scienceen_US


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