• Login
    View Item 
    •   etd@IISc
    • Division of Chemical Sciences
    • Inorganic and Physical Chemistry (IPC)
    • View Item
    •   etd@IISc
    • Division of Chemical Sciences
    • Inorganic and Physical Chemistry (IPC)
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Studies on Oxovanadium(IV) Complexes for Photocytotoxicity and Cellular Imaging

    View/Open
    Thesis(Abstract) (44.01Kb)
    Thesis(Full Text) (17.93Mb)
    Author
    Banerjee, Samya
    Metadata
    Show full item record
    Abstract
    The thesis work deals with different aspects of the chemistry of oxovanadium(IV) complexes, their interaction with DNA, photo-induced cleavage of double-stranded DNA, photocytotoxic effect on cancer and normal cells in visible light, mechanism of cell death, cellular uptake and localization behavior. Chapter I presents a general introduction to cancer, its different treatment modalities, organic molecules and transition metal complexes as photo-chemotherapeutic agents. Various modes of interactions of small molecules, capable of targeting DNA leading to DNA strand scission, emphasizing particularly the photo-induced DNA cleavage activities are discussed for their potential application in PDT. The potential and the prospects of metal-based photoactivated chemotherapy (PACT) are discussed in brief. Objective of the thesis work is also dealt in this Chapter. Chapter II of the thesis deals with the synthesis, characterization, DNA binding and near-IR red light-induced DNA cleavage activity of ternary oxovanadium(IV) complexes of polypyridyl ligands and phenanthroline bases to explore the red light-induced DNA cleavage activity and photocytotoxicity in visible light in HeLa cells. Chapter III presents the synthesis, characterization, DNA binding and near-IR light-induced DNA cleavage activity, cellular uptake, visible light assisted photocytotoxicity in different cancer cells, mode of photo-induced cell death and cellular localization of ternary oxovanadium(IV) complexes containing curcumin or diglucosylcurcumin and phenanthroline bases. The main objectives of this work are (i) to make our complexes fluorescent for cellular imaging and (ii) to enhance the solubility and tumor targeting potential of the complexes. Chapter IV describes the synthesis, characterization, DNA binding, red-light induced DNA cleavage activity, photocytotoxicity in normal and cancer cells, mode of light assisted cell death and cellular localization of ternary oxovanadium(IV) complexes having anacac/curcumin and pyridyl phenanthroline base with an objective to enhance the stereo-chemical rigidity and to improve the photoactivity of the complexes by introducing an additional pyridyl group. Chapter V deals with the synthesis, characterization, DNA binding and photo-induced DNA cleavage activity and photocytotoxicity of the oxovanadium(IV) complexes of curcumin or diglucosylcurcumin ligands and acdppz base with the aim to augment the photocytotoxicity of the dppz complexes. Cell death mechanism and confocal microscopic studies are also carried out to gain more insight into the PDT effect caused by light in the presence of the complexes and the results are compared with the clinically used drug Photofrin®. In Chapter VI we have designed the ternary oxovanadium(IV) vitamin-B6 Schiff base complexes of acdppz to achieve vitamin B6 transporting membrane carrier (VTC) mediated entry of these complexes into the tumour cells in preference to the normal ones. The complexes were synthesized, characterized and their cellular uptake, photocytotoxicity, mode of photo-induced cell death in various cancer and normal cells studied. Endoplasmic reticulum targeting photocytotoxic property of the complexes has been studied in details. Finally, the summary of the dissertation and conclusions drawn from the present investigations are presented. The references in the text have been indicated as superscript numbers and assembled at the end of each chapter. The complexes presented in this thesis are represented by bold-faced numbers. Crystallographic data of the complexes which are characterized structurally by single crystal X-ray crystallography are provided in CIF format in the enclosed CD (Appendix-I). Due acknowledgements have been made wherever the work described is based on the findings of other investigators. Any omission that might have happened due to oversight or mistake is regretted.
    URI
    https://etd.iisc.ac.in/handle/2005/4074
    Collections
    • Inorganic and Physical Chemistry (IPC) [304]

    Related items

    Showing items related by title, author, creator and subject.

    • Syntheses and Structure Elucidations of Ternary Metal (Cu/Co)Complexes with Nucleic Acid Constituents 

      Prakash, Patil Yogesh (2018-05-07)
      The thesis is divided into four chapters Chapter 1 provides a brief introduction to the metal-nucleic acid interactions, the role of synthetic models to understand them with both solution (potentiometric) and solid state ...
    • Studies on Near-IR Light Photocytotoxic Oxovanadium Complexes 

      Prasad, Puja (2018-04-11)
      The present thesis deals with different aspects of the chemistry of oxovanadium(IV) complexes, their interaction with double stranded DNA, photo-induced DNA cleavage, photo-enhanced cytotoxicity in visible light and red ...
    • Targeted Delivery of Cytotoxic Metal Complexes into Cancer Cells with and without Macromolecular Vehicles 

      Mitra, Raja (2018-04-12)
      Anticancer active metal complexes such as cisplatin are routinely used for treating various cancers since 1978. However, the side effects of cisplatin overwhelm its therapeutic potential, especially in the latter stages ...

    etd@IISc is a joint service of SERC & J R D Tata Memorial (JRDTML) Library || Powered by DSpace software || DuraSpace
    Contact Us | Send Feedback | Thesis Templates
    Theme by 
    Atmire NV
     

     

    Browse

    All of etd@IIScCommunities & CollectionsTitlesAuthorsAdvisorsSubjectsBy Thesis Submission DateThis CollectionTitlesAuthorsAdvisorsSubjectsBy Thesis Submission Date

    My Account

    LoginRegister

    etd@IISc is a joint service of SERC & J R D Tata Memorial (JRDTML) Library || Powered by DSpace software || DuraSpace
    Contact Us | Send Feedback | Thesis Templates
    Theme by 
    Atmire NV