Browsing Biochemistry (BC) by Subject "Research Subject Categories::NATURAL SCIENCES::Chemistry::Biochemistry"
Now showing items 1-20 of 46
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Biochemical characterization of UvrD helicase and its 1 interplay with DNA mismatch repair proteins in 2 Neisseria gonorrhoeae
UvrD helicase belongs to type I superfamily of helicases. It participates in Nucleotide Excision Repair (NER) and DNA Mismatch Repair (MMR). It also participates in the rolling circle replication. It has been shown that ... -
Conservation and divergence of the nucleotide excision repair pathway: Comparison of UvrB and UvrC proteins from Mycobacterium tuberculosis and Escherichia coli reveal significant differences in their functional properties
This thesis is presented in three chapters divided by topics. Chapter I will begin with an overview of literature regarding different types of DNA damage, DNA repair pathways in bacteria, properties of E. coli UvrABC ... -
Deciphering the biochemical and biophysical properties of the Holliday junction resolvases RuvC and RuvX from Mycobacterium smegmatis
Homologous recombination (HR) is a ubiquitous cellular process that occurs in all three domains of life as well as in DNA and RNA viruses. In eukaryotes, HR is critically important for homology-directed DNA repair (HDDR) ... -
Determination of significance of SUMOylation of the Smc5/6 complex
The fascination of scientists to the Smc5/6 complex began in the 1990s, with the characterization of UV and γ radiation-sensitive rad18 mutant and its identification as a component of a novel Structural Maintenance of ... -
Development and Characterization of a potent Tumor Necrosis Factor-alpha blocking agent
Tumor necrosis factor-α (TNFα), one of the major pro-inflammatory cytokines, plays a pivotal role in an effective inflammation process and immune response. TNFα interacts with two different receptors, TNFR1 and TNFR2, which ... -
The dual-targeting of Saccharomyces cerevisiae Pso2 into the nucleus and mitochondria is regulated by post-translational modifications: Implications for organelle-specific DNA repair
The repair of DNA interstrand crosslinks (ICLs) involves the concerted action of multiple DNA repair pathways. In eukaryotes, ICLs are sensed and repaired by replication-coupled or replication-independent mechanisms in ... -
Establishment of human ectopic liver tissue in immunocompromised mice as a model for Hepatitis C Virus studies
Infection with hepatitis C virus (HCV) leads to an inflammatory condition which may progress to liver cirrhosis and in some cases, hepato-cellular carcinoma. Though, new drugs are being added to the list of HCV therapeutics, ... -
Exploring the Marine Algicolous Endophytic Fungi: A Promising Source of Novel Cancer Therapeutics
Cancer is the second leading cause of mortality globally, with over 6 million new cases diagnosed annually. Research on effective therapies is crucial due to the disease's complexity. Natural materials in chemotherapy have ... -
Exploring the role of low complexity protein sequence in regulating RNA granule dynamics and translation control
RNA granules are conserved membraneless mRNP complexes that play an important role in determining mRNA fate by affecting translation repression and mRNA decay. Processing bodies (P-bodies) harbor enzymes responsible for ... -
Functional characterization of Salmonella Typhimurium encoded Cold Shock Protein E during stress responses, biofilm formation and virulence
Overall, this study reports novel functional roles of S. Typhimurium encoded CspE during bile resistance, biofilm formation and in vivo virulence. It also establishes importance of an uncharacterised protein YciF, implicating ... -
Identification and Biochemical Characterization of Novel Inhibitors of Nonhomologous End Joining: Implications in DNA Double-Strand Break Repair and Cancer Therapy
To summarize, in the present study, we have synthesized and characterized three novel NHEJ inhibitors targeting DNA Ligase IV, with several fold better efficacy compared to SCR7 and thus potential to be developed as cancer ... -
In vivo mechanistic insights into the trans-splicing based expression of Hsp90 in Giardia lamblia
Giardia lamblia, a simple yet intriguing protozoan parasite, challenges conventional paradigms in gene expression through its unique mechanism of trans-splicing for Heat Shock Protein 90 (Hsp90). As a primitive organism, ... -
Insights into life-cycle stage transition of Trichomonas vaginalis and advancement in its diagnosis
Trichomonas vaginalis is a protozoan parasite and the causative agent of the most common non-viral, sexually transmitted disease (STD) in humans known as trichomoniasis. WHO estimates more than 270 million cases of ... -
Insights into the Differential Cytotoxicity of Abrin and Design of a Vaccine Candidate against the Toxin
Ribosome inactivating proteins (RIPs) from plants, fungi or bacteria are extremely lethal due to their N-glycosidase activity on eukaryotic ribosomal RNA. As the name indicates, these toxins inactivate ribosomes; thereby ... -
Investigating DNA structural basis for fragility during chromosomal translocations associated with human B-cell lymphomas
Genome integrity is essential for normal cellular functions. Erroneous repair of DNA double-strand breaks (DSBs) could lead to chromosomal rearrangements, including chromosomal translocations that may result in altered ... -
Investigation of RAG1 in lymphoid and brain cells: Understanding mechanism and regulation
In summary, we show that RAG1 expression is regulated through a novel miRNA (miR- 29c) mediated mechanism and establish that as an additional mode of RAG1 regulation. Studies described in the thesis reveal that among the ... -
Isolation, structure elucidation and increasing anticancer efficacy of an anti-cancer secondary metabolite from a marine-derived endophytic fungus, Aspergillus species
Cancer is the leading cause of mortality globally, contributing to ~10 million fatalities in 2020, or roughly one in every six deaths, according to global cancer statistics 2020. Around 50% of all small molecules approved ... -
Kinetic, Catalytic and Epigenetic Properties of HpyAXVII DNA Methyltransferase – a Phasevarion in the Human Pathogen Helicobacter pylori
Helicobacter pylori is the leading cause of gastric cancer in human beings. It is a helix-shaped, Gram-negative, motile bacteria that colonizes the gastric mucosa and eventually the gastric epithelium. It has an overabundance ... -
A Ligase IV/XRCC4-dependent single strand break repair pathway for the maintenance of A/T-rich regions during DNA replication in mammals
The DNA in our cells is under constant threat from various exogenous and endogenous sources. To ensure error free and faithful transmission of hereditary material, human cells employ several DNA repair pathways. Mammalian ... -
Mechanistic insights into the replication of Sesbania mosaic virus
The present thesis deals with the mechanism of replication of Sesbania mosaic virus (SeMV), which belongs to the genus sobemoviruses. Sobemoviruses are single stranded positive sense RNA viruses which infect both mono and ...