Show simple item record

dc.contributor.advisorDas, Puspendu K
dc.contributor.authorMishra, Akriti
dc.date.accessioned2022-11-11T10:28:06Z
dc.date.available2022-11-11T10:28:06Z
dc.date.submitted2021
dc.identifier.urihttps://etd.iisc.ac.in/handle/2005/5906
dc.description.abstractNanoparticle-based biomedical applications in disease diagnostics, site targeting, drug delivery, etc., have been pursued vigorously with the hope of finding better solutions than conventional diagnostics and therapeutics. During any such application the nano surface will get exposed to complex biological fluids which may lead to the adsorption of layers of proteins (or other biomolecules), popularly known as “Nanoparticle-protein corona (NPC)”. Proteins having high affinity and longer residence time on the surface form hard corona (first layer) while soft corona (subsequent layers) is formed by proteins that interact weakly with the first adsorbed layer on the surface. Although a few nanoformulations have been approved for human trial, enormous challenges persist for their safe and successful implementation, mainly due to poor understanding of the energetics associated with the stability of NPC formation. The mechanism of protein adsorption on nanoparticles is thus essential for their safe use in nanomedicine and novel nano formulations. In my thesis work, I have employed mainly second harmonic light scattering (SHLS) and dynamic light scattering (DLS) techniques in solution to probe the thermodynamics of adsorption of proteins on biocompatible gold and silica nanoparticles. The important thermodynamic parameters like binding constant, binding stoichiometry, Gibbs free energy (G), enthalpy (H), and entropy (S) of NPC formation were all extracted after fitting the scattering data with various adsorption modelsen_US
dc.language.isoen_USen_US
dc.rightsI grant Indian Institute of Science the right to archive and to make available my thesis or dissertation in whole or in part in all forms of media, now hereafter known. I retain all proprietary rights, such as patent rights. I also retain the right to use in future works (such as articles or books) all or part of this thesis or dissertationen_US
dc.subjectNanoparticle-protein coronaen_US
dc.subjectdynamic light scatteringen_US
dc.subjectGolden_US
dc.subjectSilveren_US
dc.subjectNanoparticlesen_US
dc.subject.classificationResearch Subject Categories::NATURAL SCIENCES::Chemistry::Inorganic chemistryen_US
dc.titleThermodynamics of Protein Adsorption on Gold and Silica Nanoparticlesen_US
dc.typeThesisen_US
dc.degree.namePhDen_US
dc.degree.levelDoctoralen_US
dc.degree.grantorIndian Institute of Scienceen_US
dc.degree.disciplineFaculty of Scienceen_US


Files in this item

This item appears in the following Collection(s)

Show simple item record