| dc.contributor.advisor | Raghavan, Sathees C | |
| dc.contributor.author | Ghosh, Dipayan | |
| dc.date.accessioned | 2022-09-06T11:14:21Z | |
| dc.date.available | 2022-09-06T11:14:21Z | |
| dc.date.submitted | 2022 | |
| dc.identifier.uri | https://etd.iisc.ac.in/handle/2005/5847 | |
| dc.description.abstract | This study has provided new insights into the mechanistic details of NHEJ
and showed that DNA sequence around the break site can be a factor in determining which
proteins involved in NHEJ might be recruited with a higher efficiency. We have also generated
a KU70-mutant Nalm6 cell line (N6-Ku70-2) using CRISPR-Cas9 genome editing technology
and explored the differences between these cells and WT Nalm6 cells in terms of cell
proliferation and DNA repair potential. Finally, we have investigated the importance of
RNASEH2A/RNASEH2B expression in the maintenance of proper DSB repair and cellular
viability in T-ALL cells. | en_US |
| dc.language.iso | en_US | en_US |
| dc.rights | I grant Indian Institute of Science the right to archive and to make available my thesis or dissertation in whole or in part in all forms of media, now hereafter known. I retain all proprietary rights, such as patent rights. I also retain the right to use in future works (such as articles or books) all or part
of this thesis or dissertation | en_US |
| dc.subject | DNA sequence | en_US |
| dc.subject | nonhomologous end joining | en_US |
| dc.subject | DNA double-strand breaks | en_US |
| dc.subject | DSB substrates | en_US |
| dc.subject | KU70 gene | en_US |
| dc.subject | Nalm6 cells | en_US |
| dc.subject.classification | Research Subject Categories::NATURAL SCIENCES::Chemistry::Biochemistry | en_US |
| dc.title | Understanding Nonhomologous DNA End Joining: Novel Insights Into Mechanism and Regulation | en_US |
| dc.type | Thesis | en_US |
| dc.degree.name | PhD | en_US |
| dc.degree.level | Doctoral | en_US |
| dc.degree.grantor | Indian Institute of Science | en_US |
| dc.degree.discipline | Faculty of Science | en_US |
