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dc.contributor.advisorSubba Rao, P V
dc.contributor.authorShenai, Bhaskar R
dc.date.accessioned2005-04-29T05:30:07Z
dc.date.accessioned2018-07-30T14:15:18Z
dc.date.available2005-04-29T05:30:07Z
dc.date.available2018-07-30T14:15:18Z
dc.date.issued2005-04-29T05:30:07Z
dc.date.submitted1994-08
dc.identifier.urihttp://etd.iisc.ac.in/handle/2005/112
dc.identifier.srnonull
dc.description.abstractA crude extract of axenically grown amoebae was used as antigen in order to develop an AB microELISA for the detection and quantitation of E. histolytica-specific IgG antibodies. This ELISA was used to screen individual sera of patients suffering from invasive amoebiasis (n=47)and control individuals(n=33). Significant titers of E-histolytica- specific IgG antibodies were present only in sera of patients suffering from invasive amoebiasis. The AB-microELISA had a sensitivity of 98% and a specificity of 97%. Immunoblot analysis of the crude E. histolytica extract indicated the presence of several antigenic proteins. One of the common antigenic proteins recognized by the individual patients' sera had a molecular weight of 160.170 kDa Sera of five patienta with high titers of E. histotytica-specific IgG antibodies were used to prepare a serum pool. This pooled serum was used for immunoscreening of an E. histolyitca cDNA expression library prepared in the phage vector l ZAP-II. A strongly immunoreactive phage done was identified, from which he recombinant phagemid was released by in vivo excision for characterization of the cDNA insert.en
dc.format.extent4611050 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.publisherIndian Institute of Scienceen
dc.rightsI grant Indian Institute of Science the right to archive and to make available my thesis or dissertation in whole or in part in all forms of media, now hereafter known. I retain all proprietary rights, such as patent rights. I also retain the right to use in future works (such as articles or books) all or part of this thesis or dissertation.en
dc.subject.classificationLimnologyen
dc.subject.keywordinvasive amoebiasisen
dc.subject.keywordCloning-Invasiveen
dc.subject.keywordE-histolyticaen
dc.titleCloning and Expression of a Diagnostic Antigen for Invasive Amoebiasisen
dc.typeElectronic Thesis and Dissertationen
dc.degree.namePhDen
dc.degree.levelDoctoralen
dc.degree.grantorIndian Institute of Scienceen
dc.degree.disciplineFaculty of Scienceen


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