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Structural insights into assembly and regulation of HigBA toxin-antitoxin system from Escherichia coli
In the last few decades, bacterial Toxin-antitoxin (TA) systems have been identified to play crucial roles in bacterial survival under stressful conditions and virulence. TA systems are pair of genetic elements where one ...
Mutational sensitivity of CcdB mutants in their native operonic context
Determining the precise effects of thousands of point mutants on protein structure, stability, and activity is a challenging task. Chapter 1 introduces how next-generation sequencing technologies exploit phenotypic screens ...
Roles of N10-formyl-tetrahydrofolate (N10-fTHF), ribosomal large subunit pseudouridine synthase D (RluD), and transcription-translation coupling in the fidelity of translation initiation in Escherichia coli
Protein synthesis is a fundamental process in gene expression. It involves four basic steps – initiation, elongation, termination, and ribosome recycling in bacteria. However, the step of initiation is the most regulated ...
Crystallographic and analytical ultracentrifugation studies of the E. coli acetohydroxy acid synthase I enzyme
This thesis ellucidates the mechanism of activation and regulation of AHAS I
enzyme from Escherichia coli that was characterised using crystallographic and
sedimentation velocity analytical ultracentrifugation studies.
AHAS ...
Deciphering the evolutionary significance of maintaining cryptic genetic systems by bacteria
Cryptic genes are phenotypically silent DNA sequences with the potential to code for a function, but remain inactive during the normal life span of the organism. However they can be activated by a single mutational event ...
Regulation of RecA nucleoprotein filament stability by RecX and the effects of RecA-membrane interaction on the activities of RecA in mycobacteria
E. coli RecA is a multifunctional protein known to be associated with the cell membrane, forming foci often located at the cell poles, which gets redistributed along the length of the cell during SOS response. Several lines ...