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Regulation of Flagellar Motility in Giardia lamblia and Trichomonas vaginalis
Protozoan pathogens are responsible for infections that are highly prevalent, especially in developing and under-developed countries. Flagellar motility is exhibited by a variety of organisms ranging from bacteria to certain ...
Mechanistic insights into protein stabilization and protein-protein interactions
Most amino acid substitutions in a protein either lead to partial loss of function or are near neutral. Several studies have shown the existence of second-site mutations that can rescue the defects caused by diverse loss ...
Studies on the roles of rffG and rfbB encoding dTDP-glucose 4,6- dehydratase in Salmonella Typhimurium
Salmonellosis is a major health concern which causes significant morbidity and mortality worldwide. Infection with Salmonella Typhimurium leads to self-limiting diarrhea in healthy individuals and invasive disease in ...
Probing Protein Sequence-Function Relationships using Deep Mutational Scanning
Deep Mutational Scanning (DMS) approaches help elucidate sequence-function-phenotype relationships in proteins, which ultimately improves our understanding of residue (or nucleotide)-specific contributions to protein ...
Structural and functional insights of biological macromolecules associated with infectious diseases – single particle cryo-electron microscopy based studies
In the global context, particularly in India, the two most important infectious diseases among many others are Tuberculosis (TB), an age-old disease and COVID-19, a new-age disease. This thesis focuses on some of the key ...
Immunological consequences of host signaling-regulated epigenetic modification(s) during mycobacterial pathogenesis
Introduction: Mycobacterium tuberculosis (Mtb), the principal etiological agent of pulmonary tuberculosis (TB), continues to co-evolve with the human population making itself one of the most potent infectious killer in the ...
Roles of Protein Acetylation in the Human Pathogen Helicobacter pylori
Helicobacter pylori is a gram-negative epsilon proteobacterium infecting half of the world population. H. pylori is a naturally competent bacterium with a huge repertoire of Restriction-Modification (RM) systems. The ...
Structural, mechanistic and pharmacological studies of inhibitory neurotransmitter transporters
Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the central nervous system (CNS). The synaptic levels of GABA are controlled by the activity of GABA transporters (GATs) present in the presynaptic ...
Characterization of the transsulfuration enzyme Cystathionine beta-synthase as a novel regulatory node in the sulfur metabolism of Mycobacterium tuberculosis
Methionine and cysteine metabolisms are important for the survival and pathogenesis of Mycobacterium tuberculosis (Mtb). The transsulfuration pathway converts methionine to cysteine and represents an important link between ...
Structural and Biophysical studies to evaluate the influence of oligomerization on biomolecular function
Oligomeric proteins are more common than monomers. This aspect of molecular evolution substantially contributes to functional diversity and the acquisition of features such as cooperativity and regulatory mechanisms. Not ...