Browsing Division of Biological Sciences by Subject "Research Subject Categories::NATURAL SCIENCES::Biology::Cell and molecular biology"
Now showing items 21-40 of 77
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Establishment of a knock-in mouse model expressing a hypomorphic variant of receptor guanylyl cyclase C
Receptor guanylyl cyclase C (GC-C, gene GUCY2C) is expressed on the apical surface of the intestinal epithelial cells and gets activated by the endogenous ligands guanylin and uroguanylin. Heat-stable enterotoxin (ST) ... -
First thorough characterization of the structure, interactions and specificity of an archeal lectin with implication for TB infection
Decades long studies on plant lectins carried out in this laboratory have contributed substantially to glycobiology and helped in the initiation and development of macromolecular crystallography in India. Subsequently the ... -
Functional and structural studies of proteins involved in telomere maintenance
The linear ends of the eukaryotic chromosomes are protected by a specialized nucleoprotein complex known as telomere. Telomeres consist of telomere DNA and telomere DNA binding proteins (known as shelterin complex). The ... -
Functional characterisation of splicing factor Slu7 in Cryptococcus neoformans – roles in cell cycle, constitutive and alternate splicing
Fungal genomes, including in pathogenic yeasts like Cryptococcus neoformans (Cnn) and Candida albicans are rich with short introns. 99% of Cryptococcus neoformans genes have introns with an average of 5 introns per gene ... -
Functional characterization of a new enzymatic activity of the ‘miRNase’-XRN-2 from Caenorhabditis elegans.
Ribonucleic acid (RNA) molecules play a central role in every pivotal process in the cell, and ribonucleases (RNases) are critical for their biogenesis, processing, and degradation. Therefore, RNases are indispensable for ... -
Functional characterization of WD40-repeat protein, WDR8, in Zebrafish to gain insight into its role in Isolated Microspherophakia
WD40-repeat (WDR) proteins are a family of proteins that are characterized by widespread occurrence, low level of sequence conservation, common structural conformation (β propeller structure) and functional diversity. ... -
Genetics of Glioblastoma: Insights into Biology and Therapy Resistance
The central nervous system contains the neural stem cells which differentiate and give rise to the neurons as well as the glial cells which are non-neuronal in nature and provide support and protection to neurons. Unlike ... -
Homeostasis of cyclic-di-AMP in Mycobacterium smegmatis: Functional and structural contributions of c-di-AMP synthase (MsDisA) and hydrolase (MsPDE)
In bacteria, cyclic-di-nucleotide based second messengers regulate various physiological processes including the stress response. For the past decades, cyclic diadenosine monophosphate (c-di-AMP) has emerged as a crucial ... -
Identifying key determinants underlying variable efficacy of BCG substrains against Pulmonary TB.
Protection conferred by BCG vaccine against adult pulmonary TB ranges from 0–80% based on large, well-controlled field trials. This variability is highly significant (P < 0.0001) and is regarded as being indicative of true ... -
Immunological consequences of host signaling-regulated epigenetic modification(s) during mycobacterial pathogenesis
Introduction: Mycobacterium tuberculosis (Mtb), the principal etiological agent of pulmonary tuberculosis (TB), continues to co-evolve with the human population making itself one of the most potent infectious killer in the ... -
Improving Glioblastoma Management: A Study of Biomarkers, Tumor Microenvironment and Chemoresistance
Glioblastoma (GBM) is an aggressive form of adult brain tumors. The current standard of care includes maximal safe resection followed by concurrent chemo-radiation therapy and adjuvant chemotherapy with temozolomide. ... -
Influence of Tgf-B on Mechanobiology of Human Breast Cancer Cells
Breast cancers are characterized by extensive modifications to mechanical properties of the tissue via increased ECM production and vascularization. TGF-B is a multi-functional cytokine that is implicated in cancer invasion ... -
Integrative modeling of NS3 helicase function with molecular kinetics, molecular simulations and machine learning
The viral NS3 helicase is a crucial enzyme involved in the RNA replication of positive strand ssRNA viruses of the Flaviviridae family. The NS3 is an active motor that binds RNA and couples ATP hydrolysis to the mechanical ... -
Interactions between host cellular factors and Rotaviral viroplasmic non-structural proteins NSP5, NSP2 and their influence on virus replication
Overall, the present study demonstrates the dynamic nature of rotavirus viroplasm and how it manipulates the intracellular environment by intracellular relocalization of host proteins and their sequestration in viroplasms ... -
Manipulating Bacterial and Host Reactive Oxygen Species (ROS)- based mechanisms to potentiate killing of Mycobacterium tuberculosis (Mtb)
Mycobacterium tuberculosis (Mtb) is evolutionarily equipped to resist exogenous reactive oxygen species but shows vulnerability to an increase in endogenous ROS (eROS). Since eROS is an unavoidable consequence of aerobic ... -
Mechanistic insights into the role of miR‐6741‐3p in oral squamous cell carcinoma pathogenesis
Many functional studies and clinical analysis have linked miRNA dysregulation as a causative factor for tumor initiation and progression. Overexpression of oncogenic miRNAs and downregulation of tumor suppressor miRNAs are ... -
miRNA-based regulation of Coxsackievirus B3 replication and pathogenesis
Coxsackievirus B3 (CVB3) is a single-stranded, positive-sense RNA virus known to cause acute myocarditis, pancreatitis, and aseptic meningitis. In many cases, the long-term impact of CVB3 infection-induced cell death ... -
Molecular Characterization of Mannose Binding Lectins
Lectins are proteins of non-immunologic origin that bind to carbohydrates with high fidelity. They constitute a large class of multivalent recognition molecules that specifically interact with their cognate sugar moieties ... -
Molecular insights into AMP-activated protein kinase (AMPK)-mediated regulation of anoikis resistance
Our study identifies negative cross-talk between AMPK and two important cellular kinases, Akt and Erk. AMPK-mediated inhibition of these two kinases promotes survival of cancer cells in the matrix-deprived condition. ... -
Molecular pathways governing maturation and decay of precursor piRNAs in Caenorhabditis elegans
Piwi-interacting RNAs (piRNAs) are an animal-specific class of germline-enriched small non-coding RNAs that play a crucial role in germline development and fertility in diverse organisms. In Caenorhabditis elegans (C. ...