Browsing by Advisor "Varadarajan, Raghavan"
Now showing items 1-20 of 43
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CcdB : Stability, folding and application to design novel antibacterials
The primary amino acid sequence typically dictates the ultimate conformation of the protein. Mutations in the sequence demonstrate neutral, positive or negative effects on the structure-function relationship of the protein. ... -
Computational Analyses of Protein Structure and Immunogen Design
(2018-08-20)The sequence of a polypeptide chain determines its structure which in turns determines its function. A protein is stabilized by multiple forces; hydrophobic interaction, electrostatic interactions and hydrogen bond formation ... -
Computational And Experimental Studies On Protein Structure, Stability And Dynamics
(2014-08-18)The work in this thesis focuses on the study of three main aspects of proteins, viz, Protein structure, stability, and dynamics. Chapter 1 is a general introduction to the topics studied in this thesis. Chapter 2 deals ... -
Conformational Analysis And Design Of Disulfides In Antiparallel β-Sheets And Helices
(2013-08-30)Disulfides are the primary covalent interactions within a protein molecule that connect residues which are sequentially distant. Naturally occurring disulfides enhance the stability of the protein by destabilization of ... -
Deep Mutational Scanning Analyses of Protein Stability and Function
Protein stability is critical to understanding protein function, folding, and interactions within living organisms. It plays a key role in protein engineering, where designing stable proteins is essential for developing ... -
Deep mutational scanning to enhance immunogenicity and map antibody responses to HIV-1
Embargo upto 15/7/2025 -
Design and Application of Temperature Sensitive Mutants in Essential Factors of RNA Splicing and RNA Interference Pathway in Schizosaccharomyces Pombe
(2018-05-10)Gene deletions are a powerful method to uncover the cellular functions of a given gene in living systems. A limitation to this methodology is that it is not applicable to essential genes. Even for non-essential genes, gene ... -
Design and Characterization of HIV-1 ENV Derived Immunogens
(2018-06-21)The Human Immunodeficiency Virus (HIV) is a member of the retroviridae family from lentivirus genus which primarily infects CD4+ T cells and also to lesser degree monocytes, macrophages, and dendritic cells causing progressive ... -
Design and characterization of stabilized mutants of the SARS-CoV-2 Receptor Binding Domain
The global pandemic caused by SARS-CoV-2 has resulted in millions of deaths worldwide, highlighting the urgent need for effective vaccines. The trimeric spike glycoprotein, particularly the receptor-binding domain (RBD), ... -
Design And Isolation Of Temperature Sensitive Mutants Of Gal4 In Yeast And Drosophila
(2009-04-16)Genomic and proteomic investigations have yielded, and continue to produce, a large amount of information about genes and their protein products. In contrast, the evidence bearing on physiological roles of specific proteins ... -
Design and Stabilization of Stem Derived Immunogens from HA of Influenza A Viruses
(2018-07-19)Influenza virus belongs to the Orthomyxovirus family of viruses that causes respiratory infection in humans, leading to morbidity and mortality. The mature influenza A virion has an envelope that contains two major surface ... -
Design of Immunogens Derived from the Conserved Stem of Influenza Virus Hemagglutinin
Embargo up to 15/7/2025 -
Design of Influenza Immunogens by Hemagglutinin (HA) Protein Minimization
(2018-01-31)Influenza virus is a pleiomorphic human pathogen which causes self-limiting respiratory illness lasting one-two weeks in most individuals. However, in immunologically compromised individuals, influenza infection may lead ... -
Design of soluble and particulate immunogens derived from the stem of Influenza hemagglutinin
Influenza is a highly contagious virus, belonging to the family Orthomyxoviridae that causes acute febrile respiratory illness which can be fatal in some cases. The highest risk groups for influenza viral infection ... -
Design, Biophysical Characterization, and Serum Epitope Mapping of SARS-CoV-2 RBD Vaccine Immunogens
Embargo up to Jan 9, 2026 -
Determinants Of Globular Protein Stability And Temperature Sensitivity Inferred From Saturation Mutagenesis Of CcdB
(2011-02-25)The unique native structure is a basic requirement for normal functioning of most proteins. Many diseases stem from mutations in proteins that destabilize the protein structure thereby resulting in impairment or loss of ... -
Determinants of protein stability and function inferred from saturation mutagenesis and next-generation sequencing
Proteins play a central role in all biological processes. Despite a similar underlying composition, they exhibit immense diversity in structures and functions, a subject of great interest. The research presented in this ... -
Development of high throughput methodologies to isolate stabilized mutants and to identify interfacial residues
When eukaryotic or unstable proteins are overexpressed in bacteria, these often form insoluble aggregates called inclusion bodies (IB). Refolding the proteins from inclusion bodies can be challenging, resulting in low ...