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    Browsing by Advisor "Varadarajan, Raghavan"

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    Now showing items 21-35 of 35

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      • Modulation of Protein Stability and Function by Cysteine Mutations and Signal Peptides 

        Sharma, Likhesh (2018-06-23)
        Chapter 1gives a general introduction to the CXXC motif found in natural proteins. It then reviews the studies where disulphides were engineered in various proteins. The various strategies developed to engineer metal binding ...
      • Molecular Characterization of Mannose Binding Lectins 

        Nisha, G J
        Lectins are proteins of non-immunologic origin that bind to carbohydrates with high fidelity. They constitute a large class of multivalent recognition molecules that specifically interact with their cognate sugar moieties ...
      • Molecular Determinants of Mutant Phenotypes in the CcdAB Toxin -Antitoxin System 

        Guptha, Kritika (2018-05-29)
        A major challenge in biology is to understand and predict the effect of mutations on protein structure, stability and function. Chapter 1 provides a general introduction on protein sequence-structure relationships and use ...
      • Mutational sensitivity of CcdB mutants in their native operonic context 

        Bajaj, Priyanka
        Determining the precise effects of thousands of point mutants on protein structure, stability, and activity is a challenging task. Chapter 1 introduces how next-generation sequencing technologies exploit phenotypic screens ...
      • Probing Protein Sequence-Function Relationships using Deep Mutational Scanning 

        Chandra, Soumyanetra
        Deep Mutational Scanning (DMS) approaches help elucidate sequence-function-phenotype relationships in proteins, which ultimately improves our understanding of residue (or nucleotide)-specific contributions to protein ...
      • Protein Engineering and Stabilization of HIV-1 Envelope Glycoprotein 

        Kesavardana, Sannula (2018-05-09)
        A number of viral diseases such as Hepatitis B, small pox, measles, rubella and polio have effective vaccines to control or eradicate them. HIV-1 is a lentivirus which infects human immune cells and leads to the disease ...
      • Protein Engineering of HIV-1 Env and Human CD4 

        Saha, Piyali (2018-05-01)
        Since, its discovery over three decades ago, HIV has wrecked havoc worldwide. According to the UNAIDS report 2011, at present 34 million people is living with HIV and AIDS vaccine with broadly neutralizing activity still ...
      • Protein Minimization Of Human CD4 And Design Of gp120-CD4 Single Chain Immunogens 

        Sharma, Deepak Kumar (2011-10-19)
      • Protein Structure and Mutant Phenotype Prediction from Analysis of Mutant Libraries by Deep Sequencing 

        Khare, Shruti
        Proteins play a central role in all the biological processes. The immense diversity in protein structures and functions despite similar underlying composition is intriguing. The work presented in this thesis aims to provide ...
      • Rational Elicitation of Cold Sensitive Phenotypes 

        Chetana Baliga, B
        Conditional gene expression and conditional mutants provide a means to modulate the expression of specific genes and to control the activity of their protein products in vivo, so as to be able to study their effects on the ...
      • Scanning mutagenesis studies of HIV-1 Env immunogenicity and structure 

        Datta, Rohini
        Human Immunodeficiency Virus type 1 (HIV-1) is the etiological agent of Acquired Immunodeficiency Syndrome (AIDS). AIDS is a global pandemic with 36.7 million people living with HIV-1 all over the world and approximately ...
      • Sequence Analysis And Design Of Immunogens From The Stem Domain Of Influenza Hemagglutinin 

        Bommakanti, Gayathri (2013-07-10)
        Influenza is an important respiratory pathogen that infects several million people each year. Currently available flu vaccines have to be updated regularly in order to be effective as the virus changes its composition by ...
      • Targeting The CD4 Biniding Site In HIV-1 Immunogen Design 

        Bhattacharyya, Sanchari (2016-11-15)
        Over three decades have passed since the discovery of HIV-1, yet an AIDS vaccine remains elusive. The envelope glycoprotein of HIV-1 gp120, is the most exposed protein on the viral surface and thus serves as an important ...
      • Thermodynamic & Kinetic Characterization Of The Folding Of E.coli Maltose-Binding Protein 

        Ganesh, C. (2011-11-17)
      • Thermodynamic Characterization Of Wild Type And Mutants Of The E.coli Periplasmic Binding Proteins LBP, LIVBP, MBP And RBP 

        Prajapati, Ravindra Singh (2009-03-13)
        Native states of globular proteins typically show stabilization in the range of 5 to 15 kcal/mol with respect to their unfolded states. There has been a considerable progress in the area of protein stability and folding ...

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