https://etd.iisc.ac.in/handle/2005/7 2026-04-15T21:26:44Z 2026-04-15T21:26:44Z Guha, Sarmistha https://etd.iisc.ac.in/handle/2005/5304 2021-09-21T07:32:53Z

Altering DNA topology in mycobacteria: impact of perturbation of DNA gyrase on physiology and gene expression Guha, Sarmistha In the current dissertation, efforts have been made to probe the in vivo role of DNA gyrase to determine its importance in the growth, physiology and gene expression in mycobacteria. In this dissertation, the role of DNA gyrase in gene expression were explored. The thesis is divided into four chapters. In Chapter I, a general introduction to DNA topology, genome supercoiling, DNA topoisomerases, their mechanism of action Synopsis xvi and regulation is provided. It covers a description of the central player- DNA gyrase followed by its functions in vitro and various factors involved in the regulation of supercoiling. Further, the regulation of topoisomerase activity and the role in gene regulation has been described. In Chapter 2, the studies are aimed at generation and characterization of conditional gyrase mutant in M. smegmatis. Depletion of gyrase level beyond the 50% threshold drastically impaired cell growth and viability indicating the minimal gyrase level is required for cell sustenance. Various pleiotropic effects, altered colony morphology, elongated cells and diffused nucleoid were observed in the gyrase-depleted cells. The perturbation in the gyrase level resulted in a reduction of FtsZ levels in elongated cells suggesting the link between gyrase and cell division. Further, transcript analysis indicated gyrase as a global regulator modulating the expression of the genes involved in encoding topology modulators, transcription and core DNA transaction processes. Altered transcription pattern was a result of impaired occupancy of RNAP at the promoters and coding sequences. The gyrase knockdown strain acquired increased sensitivity to drugs used in TB treatment demonstrating its utility to screen new anti-tubercular drugs. The study thus establishes the essentiality of gyrase for mycobacterial growth, physiology and illustrates the consequences of perturbing intracellular gyrase levels on gene expression in mycobacteria. In Chapter 3, studies have been carried out to validate the in vivo functionality of the MtGyrBA fusion protein. Since gyrB and gyrA of M. tuberculosis are separated by a 34 bp stretch and transcription is controlled by the presence of 3 promoters, efforts have been directed towards constructing the fusion gyrase by linking the gyrB and gyrA genes together with a 6 bp linker sequence. The gyrBA fusion has been expressed in a mycobacterial vector under an inducible promoter. MtGyrBA rescued the growth defect showed by the M. smegmatis gyrase-depleted cells and partially complemented the E. coli ts mutants. The utility of the complemented strain has been tested to screen for drugs that target the M. tuberculosis gyrase in the background of fast growing M. smegmatis. Chapter 4 of the thesis focuses on the transcriptomic landscape of M. tuberculosis gene expression using novobiocin as an agent to bring about the relaxation of the genome at 6 hr and restoration of supercoiling due to relaxation-stimulated transcription (RST) at 24 hr of treatment. Treatment with the inhibitor changed the expression of a large number of genes. 53% of the genome exhibited relaxation-dependent transcription while 41% showed supercoiling-sensitive transcription. Genes with altered expression are distributed as large clusters across the chromosome with a distinct pattern observed during chromosome relaxation. The presence supercoil-sensitive genes interspersed between the clusters were also detected. The downregulated genes had higher AT percentage both upstream and downstream of transcription start site. Three major groups of supercoiling-sensitive genes have been identified throughout the genome: up-regulated (U), down-regulated (D) and less-responsive (LR). Thus, organization of supercoil-sensitive genes in the M. tuberculosis genome reveals DNA supercoiling as a major controller of gene expression in pathogenic mycobacteria. In conclusion, the results presented in this dissertation indicate a close connection between transcription regulation and topology by DNA gyrase mediated supercoiling

Govindan, Ashwin https://etd.iisc.ac.in/handle/2005/4119 2019-09-13T11:16:48Z

Alternate Fates of tRNAs in Initiation and Elongation Govindan, Ashwin Protein synthesis in all organisms utilizes a special tRNA called the initiator tRNA. Initiator tRNAs take part in the initiation step of protein synthesis by their direct binding to the P‐site of the ribosome. The other tRNAs (elongator tRNAs) bind first to the A‐site of the ribosome and are subsequently translocated to the P‐site during elongation. The initiator tRNA possesses sequence and structural characteristics, which enable it to perform its unique function in protein synthesis. In addition to the highly conserved three consecutive G:C base pairs in the anticodon stem of the initiator tRNA which facilitate its P‐site binding, bacterial and organellar initiator tRNAs are also formylated by FMT (methionyl‐tRNAfMet formyltransferase) to enable their binding to initiation factor 2 (IF2), directing them specifically into initiation. Structure‐function studies of E. coli initiator tRNA in‐vivo using reporter constructs showed that formylation plays a crucial role in deciding the fate of the initiator tRNA in initiation. The tRNA mutants deficient in formylation take part in initiation and/or elongation. Protein factors like IF2, elongation factor Tu (EF‐Tu) and peptidyl‐tRNA hydrolase (Pth) also contribute to the fate of the tRNA in‐vivo. The current study aims to understand how the balance of protein factors and sequence elements present on a tRNA determine its participation at the steps of initiation and/or elongation using E. coli and M. smegmatis as model organisms. The findings of my research have been described in three distinct investigations as follows: PART‐I. Development of assay systems for amber codon decoding at the steps of initiation and elongation by tRNAfMet derivatives in mycobacteria The bulk of our understanding of the mechanism of protein synthesis in bacteria is derived from the studies in E. coli. The mechanism of translation in Gram positive bacteria remains a relatively less understood process. Gram positive bacteria possess significant differences in their translational apparatus as compared to the Gram negative organisms, and therefore present with interesting systems to understand the mechanism of translation. For example, Gram positive bacteria use an indirect pathway for synthesis of Gln‐tRNAGln and Asn‐tRNAAsn as opposed to direct synthesis of Gln‐tRNAGln by glutaminyl‐tRNA synthetase (GlnRS) in E. coli. We used M. smegmatis, a slow growing Gram positive bacterium, as the model organism to study translation. The understanding of protein synthesis in these bacteria has been limited by the lack of well characterized genetic systems. Using chloramphenicol acetyltransferase (CAT) reporters (having an amber codon as the start codon or as a codon at an internal position within the reading frame of the mRNA), we developed genetic systems where the amber codon is decoded by a mutant initiator tRNA (wherein the CAU anticodon was mutated to CUA with or without additional changes in the acceptor stem) either at the step of initiation or elongation in M. smegmatis, enabling us to measure the efficiency of the mutant tRNAs in initiation or elongation in‐vivo. Characterization of the reporter encoded protein by mass spectrometric analysis showed that initiation in such a reporter proceeds through incorporation of methionine, as opposed to the use of glutamine in similar systems in E. coli. Elongation in the reporter system, carried out by formylation deficient mutants of the initiator tRNA, was found to occur through the insertion of glutamine using a two‐step pathway, where the tRNA is first aminoacylated by a non‐discriminating GluRS, followed by transamidation of the attached Glu by an amidotransferase. The formylation deficient acceptor stem mutants of the initiator tRNA were also recognized differentially by the amidotransferase in‐vivo, leading to the insertion of either glutamate or glutamine during elongation by different mutants of the tRNA. Overall, the study highlighted the conserved nature of formylation across bacteria, and its importance in the exclusive participation of the initiator tRNA in initiation. PART II. Physiological role of tRNAs that function as alternate initiator tRNAs in mycobacteria The genomes of M. tuberculosis and M. smegmatis encode three tRNAs, metU, metV and metT, with CAU anticodons. While metU has been shown to encode the initiator tRNA in these bacteria, the other two (metV and metT) are both annotated as methionine elongator tRNA. Interestingly, these tRNAs also possess some sequence characteristics like the G:C base pairs in the anticodon stem, and the lack of a Watson‐Crick base pair at 1:72 position, which are thought to be restricted to initiator tRNAs. We were interested in understanding the physiological role of such sequence elements present in the elongator tRNA. Computational and biochemical characterization of metT and metV identified them as the methionine decoding elongator tRNA (tRNAMet) and the minor form of the isoleucine decoding tRNA (tRNAIle2), respectively. Mass spectrometric analysis of metV showed that the C34 in the anticodon of the tRNA is modified to lysidine, which is consistent with its role in AUA decoding. Interestingly, the expression of metV is upregulated under stress conditions. Analysis of the modification status of the tRNA under hypoxic conditions showed that the tRNA is undermodified for lysidine, which would preclude its function in AUA decoding and enable it to function as tRNAMet. We propose that this could be a mechanism of regulation employed by cells under hypoxia. In‐vivo assays using the CAT reporter showed that metV is capable of initiating protein synthesis, providing support to our hypothesis that such tRNAs could function as alternate initiator tRNAs under stress conditions. PART III. Mitochondria‐like sustenance of E. coli on a single tRNAfMet Most organisms possess distinct methionine tRNAs that participate at the steps of initiation and elongation. However, protein synthesis in mammalian mitochondria utilizes only a single tRNAMet, which functions in both initiation and elongation. The partitioning of tRNA into initiation and elongation phases is thought to occur due to the competition between EF‐Tumt (which directs the tRNA to the elongation step) and Fmtmt (which, following formylation, directs the tRNA to the initiation step), for their binding to the single species of Met‐tRNAMet. Our studies on initiator tRNA from mycobacteria, along with the available literature on mutants of E. coli initiator tRNA, show that some mutants of initiator tRNA are capable of participating at the step of elongation. Similarly, as shown in Part‐II of the results section, native elongator tRNAs capable of initiating protein synthesis also exist in some organisms. We therefore asked if in E. coli, a single tRNAMet could participate at both the steps of initiation and elongation (as in mitochondria), and if such tRNAs fulfill the cellular needs at both steps. Using a transduction based genetic strategy, we have shown that many acceptor stem mutants of the initiator tRNA sustain E. coli for initiation and elongation function separately. Importantly, a subset of these mutants also sustains the growth of E. coli devoid of all the six copies of methionine tRNA genes (four of initiators and two of elongators). Furthermore, the mutant tRNA which is most efficient at sustaining the cell is also the one which is most like mitochondrial tRNAMet. Overall, the study provides insights into how distribution of the tRNA between FMT/IF2 and EF‐Tu determines its role in protein synthesis.

Vashistha, Surabhi https://etd.iisc.ac.in/handle/2005/1160 2025-12-03T11:08:12Z 2011-05-05T00:00:00Z

Analysis Of Interferon y-mediated Cell Cycle Arrest In Human Cancer Cells - Re-examination Of The Involvement Of Cyclin Dependent Kinase 2 Vashistha, Surabhi

2011-05-05T00:00:00Z Sondarva, Gautam V https://etd.iisc.ac.in/handle/2005/1563 2025-12-03T11:09:08Z 2011-12-12T00:00:00Z

Anticancer Drug-Induced Apoptosis Characterisation, Comparative Study And Rescue In Human Leukemic Cell Lines Sondarva, Gautam V

2011-12-12T00:00:00Z