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    Browsing by Subject "E coli"

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      • Antibacterial Surfaces Mechanisms, Design and Development 

        Singh, Jagriti
        The spread of disease-causing microorganisms through high-touch surfaces and their increased tolerance against antimicrobials and the host immune system is responsible for several fatal diseases. By the year 2050, Antimicrobial ...
      • Crystallographic and analytical ultracentrifugation studies of the E. coli acetohydroxy acid synthase I enzyme 

        Bansal, Akanksha
        This thesis ellucidates the mechanism of activation and regulation of AHAS I enzyme from Escherichia coli that was characterised using crystallographic and sedimentation velocity analytical ultracentrifugation studies. AHAS ...
      • Deciphering the evolutionary significance of maintaining cryptic genetic systems by bacteria 

        Vashishtha, Kartika
        Cryptic genes are phenotypically silent DNA sequences with the potential to code for a function, but remain inactive during the normal life span of the organism. However they can be activated by a single mutational event ...
      • Deep Mutational Scanning Analyses of Protein Stability and Function 

        Kohli, Pehu
        Protein stability is critical to understanding protein function, folding, and interactions within living organisms. It plays a key role in protein engineering, where designing stable proteins is essential for developing ...
      • Determinants of protein stability and function inferred from saturation mutagenesis and next-generation sequencing 

        Bhasin, Munmun
        Proteins play a central role in all biological processes. Despite a similar underlying composition, they exhibit immense diversity in structures and functions, a subject of great interest. The research presented in this ...
      • A molecular systems approach to unravel mechanisms and targets in antifolate-resistant bacteria and cancers 

        Bhosle, Amrisha
        Antifolates such as trimethoprim (TMP) and methotrexate (MTX) widely used for treating bacterial infections and certain cancers respectively, inhibit dihydrofolate reductase (DHFR) in the folate pathway. However, the use ...
      • Mutational sensitivity of CcdB mutants in their native operonic context 

        Bajaj, Priyanka
        Determining the precise effects of thousands of point mutants on protein structure, stability, and activity is a challenging task. Chapter 1 introduces how next-generation sequencing technologies exploit phenotypic screens ...
      • Protein folding and Disulphide rich peptide(s) folds 

        Sri Teja, Adhada
        Protein folding and protein folds are two sides of a coin. The study of protein folding processes is crucial for advancing our knowledge in fundamental cellular processes while the study of protein folds is important for ...
      • Regulation of RecA nucleoprotein filament stability by RecX and the effects of RecA-membrane interaction on the activities of RecA in mycobacteria 

        Prasad, Deepika
        E. coli RecA is a multifunctional protein known to be associated with the cell membrane, forming foci often located at the cell poles, which gets redistributed along the length of the cell during SOS response. Several lines ...
      • Roles of N10-formyl-tetrahydrofolate (N10-fTHF), ribosomal large subunit pseudouridine synthase D (RluD), and transcription-translation coupling in the fidelity of translation initiation in Escherichia coli 

        Lahry, Kuldeep
        Protein synthesis is a fundamental process in gene expression. It involves four basic steps – initiation, elongation, termination, and ribosome recycling in bacteria. However, the step of initiation is the most regulated ...
      • Roles of the ribosomal protein uS12 and the initiation factor 3 in the maintenance of fidelity of translation in Escherichia coli 

        Datta, Madhurima
        The flow of genetic information within biological systems according to the central dogma of molecular biology entails protein synthesis as the last step towards deciphering the message encrypted in the genetic code in ...
      • Structural and functional characterization of a bacterial TenpIN type III toxin-antitoxin system and its potential as an antibacterial strategy 

        Nadig, Kavyashree
        Bacterial type III toxin-antitoxin (TA) systems were first identified as abortive infection systems wherein a phage infection causes altruistic suicide of the bacterium to prevent bacteriophage spread among its clonal ...
      • Structural insights into assembly and regulation of HigBA toxin-antitoxin system from Escherichia coli 

        Jadhav, Pankaj Vilas
        In the last few decades, bacterial Toxin-antitoxin (TA) systems have been identified to play crucial roles in bacterial survival under stressful conditions and virulence. TA systems are pair of genetic elements where one ...

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