Molecular Biophysics Unit (MBU)
Recent Submissions
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Molecular Characterization of Mannose Binding Lectins
Lectins are proteins of non-immunologic origin that bind to carbohydrates with high fidelity. They constitute a large class of multivalent recognition molecules that specifically interact with their cognate sugar moieties ... -
Structural Studies on Sesbania Mosaic Virus Protease Domain and Plasmodium Falciparum Hypoxanthine Quanine Phosphoribosyl Transferase and Triosephosphate Isomerase
X-ray crystallography is a powerful tool to obtain atomic resolution details on the three-dimensional structure of biological macromolecules. Crystallographic techniques find wide applications in understanding macromolecular ... -
Role of an RNA Polymerase Interacting Protein, MsRbpA, from Mycobacterium smegmatis in Phenotypic Tolerance to Rifampicin
Rifampicin is a non-competitive inhibitor of bacterial RNA polymerase (RNAP). The knowledge about the mechanism of action of this drug has emanated from the genetic and the biochemical studies carried out on Escherichia ... -
Synthesis and Conformational Analysis of Peptides Containing the Gamma Amino Acid Residue Gabapentin
Insertion of amino acid residues into all polypeptide backbones leads to an expanded repertoire of secondary structures. In the present thesis an attempt has been made to explore the conformational properties of the ... -
Ischemic Concentrations of Lactate Increase TREK1 Channel Activity and Expression
TREK1 is a two-pore domain leak potassium channel which contributes to resting membrane potential. TREK1-/- mice exhibit increased neuronal mortality after global ischemia. Neuroprotective agents like volatile anesthetics ... -
Structural and Functional Characterization of Enzymes of Proline Biosynthesis Pathway in Mycobacterium tuberculosis
Tuberculosis is the second largest killer disease on global scale after HIVAIDS. The rapid evolution of Mycobacterium tuberculosis into drug resistant strains and the toxicity of these drugs are the major constraints in ... -
Resource allocation during Flavivirus lifecycle
Single-stranded RNA virus infection cycle progresses by resource (viral RNA) allocation to segregated macromolecular complexes: host ribosomes (translation), viral RNA polymerase (replication) and viral structural proteins ... -
Understanding intramolecular signal transduction in regulated proteases of the High Temperature Requirement A family
Protein homeostasis in all organisms is a complex process involving regulatory mechanisms that govern protein synthesis, post-translational events and degradation. The protein degradation mechanism serves multiple functions ... -
Engineering Reverse Turns: Implications in Conformation, Stability and Bioactivity of Peptides and Proteins
The work presented in this thesis provides an overview of the potential role of N-methylation in inducing reverse turns. Backbone N-methylation is one of the common modifications designed by nature and exploited by humans ... -
Structural and related studies on Mycobacterium tuberculosis pantothenate kinase
Pantothenate kinase (PanK) is a ubiquitous and essential enzyme that catalyses the first step in the universal Coenzyme A (CoA) biosynthetic pathway. In this step pantothenate is converted to phosphopantothentate, which ... -
Development and application of sequence-based approaches for recognition and functional characterization of protein kinases
Protein kinases, the third most populous protein family, are a major class of enzymes that regulate a wide range of cellular processes by phosphorylating multiple cellular proteins. There are many conserved sequence motifs ... -
Structural and Functional Studies on Salmonella typhimurium Propionate Kinase and Photorhabdus luminescens Oxalate Decarboxylase
Acetate and propionate are low molecular mass carbon compounds found abundantly in the soil. Although these compounds have been extensively used as food preservatives because of their ability to inhibit microbial growth, ... -
Structure analysis of lectin and lectin-like domains from Entamoeba histolytica
Lectins are a diverse group of proteins which can recognize different types of carbohydrates specifically and reversibly. Though first characterized in plants, lectins have now been identified in all other forms of life. ... -
Role of Second (p)ppGpp Synthetase MS_RHII-RSD in Mycobacterium smegmatis
Adaptation to a rapidly fluctuating environment is the key to the survival of an organism. Bacteria sense and respond to stress by an overall reprogramming of the cellular processes to shut down the energy-consuming processes ... -
Role of RNA Polymerase ω Subunit in Metabolism and Stress Response
RNA polymerase (RNAP) is the key enzyme in transcription and it is a multi-subunit enzyme made up of alpha, beta, beta prime and omega subunit in the stoichiometry of α2ββω. Except for the smallest subunit ω (rpoZ), all ... -
Structural Characterization of Biological Macromolecules by Random Conical Tilt Pair Experiments
Structural biology deals with determining the structure of biological macromolecules especially proteins, DNA, RNA. The conformational changes in the structure of a macromolecule helps to decipher its function. This ... -
Structural and Biochemical Studies on Mycobacterial Uracil- DNA N-glycosylase and Mut T1
Maintenance of the genomic integrity of the cell is crucial for the survival and successful propagation of an organism. However, this integrity is under continuous threat from DNA-damaging agents. In addition, errors in ... -
Protein Structure and Mutant Phenotype Prediction from Analysis of Mutant Libraries by Deep Sequencing
Proteins play a central role in all the biological processes. The immense diversity in protein structures and functions despite similar underlying composition is intriguing. The work presented in this thesis aims to provide ... -
Exploration of the Recombination Repair Pathway in Mycobacteria : Identification and Characterization of New Proteins
Genomic integrity is a fundamental requisite for survival and proliferation of all organisms. The genetic material is continuously threatened by a multitude of extrinsic and intrinsic factors. Consequently, the presence ...
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