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dc.contributor.advisorUmapathy, Siva
dc.contributor.authorMukherjee, Ria
dc.date.accessioned2020-06-03T09:50:30Z
dc.date.available2020-06-03T09:50:30Z
dc.date.submitted2019
dc.identifier.urihttp://etd.iisc.ac.in/handle/2005/4429
dc.description.abstractIn the past few decades, vibrational spectroscopy has emerged as a powerful technique in biomedical research. Raman and IR spectroscopy, which are based on signatures specific to molecular vibrations, has found a wide variety of applications ranging from fundamental research problem to applications in clinics. These two techniques combined with chemometrics can provide molecular insight to any biochemical processes. The techniques are label free, non-invasive and require minimal sample preparation allowing the study of not only simple biomolecules but also whole organisms like cells and bacteria and body fluids from human. In this thesis, important life processes including bacterial growth and stress, detection of endotoxin, prediction of metabolic disorder and DNA conformational changes has been investigated with the help of vibrational spectroscopy and chemometrics. The thesis begins with a brief introduction to Raman and IR spectroscopy and the parameters to consider for biological applications of these two techniques (Chapter 1 and 2). In the bacterial study, differences in bacterial growth have been investigated under different growth conditions with an aim to understand survival mechanism of bacteria in adverse conditions. In this thesis, the univariate, ratiometric and multivariate markers for growth under different conditions has been summarized (Chapter 3). In continuation to this, a novel resonance Raman marker band for cytochrome in E. coli has been identified. The serendipitous discovery of a new peak and its plausible applications in understanding bacterial growth and stress has been discussed (Chapter 4). Another clinically important biomolecule, which is released from the cell wall of bacteria is lipopolysaccharides (commonly known as endotoxin). Detection of endotoxin in ultralow concentration is important as these toxic substances can be lethal even when present in ng/Kg of bodyweight. Our effort to detect endotoxin with a detection limit of pg/mL concentration, with the help of Surface Enhanced Raman Spectroscopy, has been shown (Chapter 5). In the next part, potential of IR and Raman spectroscopy in studying clinically isolated human LDL samples targeting cardiovascular risk has been be demonstrated (Chapter 6). Lastly, it has been shown how Raman optical activity can be useful in understanding DNA conformational changes, a very fundamental life process (Chapter 7). Finally, the work done in the thesis and the future scope of the work has been briefed (Chapter 8). Overall, the thesis describes the applicability of vibrational spectroscopy ranging from basic research to clinics.en_US
dc.language.isoen_USen_US
dc.rightsI grant Indian Institute of Science the right to archive and to make available my thesis or dissertation in whole or in part in all forms of media, now hereafter known. I retain all proprietary rights, such as patent rights. I also retain the right to use in future works (such as articles or books) all or part of this thesis or dissertationen_US
dc.subjectInfra-Red spectroscopyen_US
dc.subjectRaman micro-spectroscopyen_US
dc.subjectResonance Raman spectroscopyen_US
dc.subjectSurface Enhanced Raman spectroscopyen_US
dc.subject.classificationResearch Subject Categories::NATURAL SCIENCES::Chemistry::Physical chemistry::Spectroscopyen_US
dc.titleRaman and IR Spectroscopic Studies of Bacteria, Endotoxin, DNA and Lipoproteinsen_US
dc.typeThesisen_US
dc.degree.namePhDen_US
dc.degree.levelDoctoralen_US
dc.degree.grantorIndian Institute of Scienceen_US
dc.degree.disciplineFaculty of Scienceen_US


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