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dc.contributor.advisorGhosh, Prosenjit
dc.contributor.authorKazi, Nayela Zeba
dc.date.accessioned2020-03-10T06:23:24Z
dc.date.available2020-03-10T06:23:24Z
dc.date.submitted2016
dc.identifier.urihttps://etd.iisc.ac.in/handle/2005/4361
dc.description.abstractBacterial population during 3.2 billion years of early earth was responsible for modifying the greenhouse gas concentration in the early earth. Here we studied the primitive facultative anaerobe E.coli K12, known to contain pathways of mixed acid fermentation in stressed oxygen deficient environment. We carried out chamber experiments where bacteria were grown in M9 minimal media containing 0.4% glucose in crimp sealed chambers for a period of 7 days-12 days. Growth rate for the bacteria were monitored using optical density measurements and CFU values on LB agar. δ13C of CO2 was analysed using Gas Bench peripheral connected with IRMS MAT 253. We observed an approximate enrichment of 3‰ in the 13C data of respired CO2 from the 1st day of incubation till 12 days of incubation under stressed conditions. We suggest that enrichment in δ13C captured a shift in the carbon source from glucose to acetate.en_US
dc.language.isoen_USen_US
dc.relation.ispartofseriesG27795;
dc.rightsI grant Indian Institute of Science the right to archive and to make available my thesis or dissertation in whole or in part in all forms of media, now hereafter known. I retain all proprietary rights, such as patent rights. I also retain the right to use in future works (such as articles or books) all or part of this thesis or dissertationen_US
dc.subjectMiller & Tans Modelen_US
dc.subjectGrowth of Microbeen_US
dc.subjectE.colien_US
dc.subjectAnaerobe E.coli K12en_US
dc.subjectBacterial Respirationen_US
dc.subject.classificationEarth Sciencesen_US
dc.titleLab Experiment Documenting Growth of Microbes in an Extreme Conditionen_US
dc.typeThesisen_US
dc.degree.nameMSc Enggen_US
dc.degree.levelMastersen_US
dc.degree.grantorIndian Institute of Scienceen_US
dc.degree.disciplineEngineeringen_US


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